Wheat variety W030258E2

ABSTRACT

A wheat variety designated W030258E2, the plants and seeds of wheat variety W030258E2, methods for producing a wheat plant produced by crossing the variety W030258E2 with another wheat plant, and hybrid wheat seeds and plants produced by crossing the variety W030258E2 with another wheat line or plant, and the creation of variants by mutagenesis or transformation of variety W030258E2. This invention also relates to methods for producing other wheat varieties or breeding lines derived from wheat variety W030258E2 and to wheat varieties or breeding lines produced by those methods.

CROSS-REFERENCE TO RELATED APPLICATIONS

The present application claims the benefit of priority to U.S.Provisional Application No. 61/874,087 filed Sep. 5, 2013, the entiredisclosures of which are hereby incorporated by reference in theirentireties.

FIELD OF INVENTION

This invention is in the field of wheat (Triticum aestivum L.) breeding,specifically relating to a wheat variety designated W030258E2.

BACKGROUND OF INVENTION

The present invention relates to a new and distinctive wheat varietydesignated W030258E2, which has been the result of years of carefulbreeding and selection in a comprehensive wheat breeding program. Thereare numerous steps involving significant technical human intervention inthe development of any novel, desirable plant germplasm. Plant breedingbegins with the analysis and definition of problems and weaknesses ofthe current germplasm, the establishment of program goals, and thedefinition of specific breeding objectives. The next step is selectionof germplasm that possess the traits to meet the program goals. The goalis to combine in a single variety an improved combination of desirabletraits from the parental germplasm. These traits may include, but arenot limited to higher seed yield, resistance to diseases and/or insects,tolerance to drought and/or heat, altered milling properties, abioticstress tolerance, improvements in compositional traits, and betteragronomic characteristics.

These processes, which lead to the final step of marketing anddistribution, can take from approximately six to twelve years ofsignificant technical human intervention starting from the time thefirst cross is made. Therefore, development of new varieties is atime-consuming process that requires precise forward planning, efficientuse of resources, and a minimum of changes in direction. The developmentof a new variety typically involves the coordinated effort of a team ofscientists, including plant breeders, molecular biologists, plantpathologists, entomologists, agronomists, biochemists,bioinformaticians, market analysts, and automation specialists.

Wheat is an important and valuable field crop. Thus, a continuing goalof wheat breeders is to develop stable, high yielding wheat varietiesthat are agronomically sound. The reasons for this goal are to maximizethe amount of grain produced on the land used and to supply food forboth animals and humans. To accomplish this goal, the wheat breeder mustselect and develop wheat plants that have the traits that result insuperior varieties.

Wheat is grown worldwide and is the most widely adapted cereal. Thereare five main wheat market classes. They include the four common wheat(Triticum aestivum L.) classes: hard red winter, hard red spring, softred winter, and white. The fifth class is durum (Triticum turgidum L.).Common wheats are used in a variety of food products such as bread,cookies, cakes, crackers, and noodles. In general the hard wheat classesare milled into flour used for breads and the soft wheat classes aremilled into flour used for pastries and crackers. Wheat starch is usedin the food and paper industries, as laundry starches, and in otherproducts. Because of its use in baking, the grain quality of wheat isvery important. To test the grain quality of wheat for use as flour,milling properties are analyzed. Important milling properties arerelative hardness or softness, weight per bushel of wheat (test weight),siftability of the flour, break flour yield, middlings flour yield,total flour yield, flour ash content, and wheat-to-flour proteinconversion. Good processing quality for flour is also important. Goodquality characteristics for flour from soft wheats include low tomedium-low protein content, a low water absorption, production oflarge-diameter test cookies and large volume cakes. Wheat glutenins andgliadins, which together confer the properties of elasticity andextensibility, play an important role in the grain quality. Changes inquality and quantity of these proteins change the end product for whichthe wheat can be used.

SUMMARY OF THE INVENTION

In certain embodiments, a plant, plant part, seed, or plant cell ofwheat variety W030258E2 is provided, representative seed of varietyW030258E2 having been deposited under ATCC accession number PTA-122841.

In certain embodiments, wheat seed is provided from the cross of theplant or plant part of wheat variety W030258E2 with a different wheatplant or plant part. Plants and plant parts grown from the seed of thecross are also provided. Methods for producing different wheat plantsare provided in which plant breeding techniques are applied to the wheatplant or plant part grown from the seed of the cross.

In certain embodiments, methods for producing progeny seed and theprogeny seed so made are provided in which a wheat plant produced bygrowing a seed of the cross of wheat variety W030258E2 with a differentwheat plant or plant part is then crossed to a plant of wheat varietyW030258E2. Methods and backcrossed seed are also provided in which theprogeny seed is grown and crossed to a plant wheat variety W030258E2 toproduce backcrossed seed.

In certain embodiments, methods for producing double haploid wheatplants are provided in which a wheat plant produced by growing a seed ofthe cross of wheat variety W030258E2 with a different wheat plant orplant part is then crossed with another plant to form haploid cells. Thechromosomes of the haploid cells are doubled to form double haploidcells which are grown into a double haploid wheat plant or plant part.

In certain embodiments, methods for cleaning, conditioning, or applyinga seed treatment to the seed of wheat variety W030258E2 are provided.

In certain embodiments, methods of milling the seed of wheat varietyW030258E2 and the flour produced from such milling is provided. Theflour may include a cell of wheat variety W030258E2.

In certain embodiments, a tissue culture of cells is provided which areproduced from the plant, plant part, seed or cell of wheat varietyW030258E2. Plants and plant parts regenerated from the tissue cultureare also provided.

In certain embodiments, wheat plants are provided which plants include atransgene and which were produced by transforming the plant, plant part,seed or cell of wheat variety W030258E2.

In certain embodiments, a plant, plant part, seed, or plant cell ofwheat variety W030258E2 further comprising a locus conversion isprovided. The plant, plant part, seed, or plant cell may have other thanthe locus conversion essentially all of the morphological andphysiological characteristics of wheat variety W030258E2. The locusconversion may confer a trait selected from male sterility, abioticstress tolerance, altered phosphorus, altered antioxidants, alteredfatty acids, altered essential amino acids, altered carbohydrates,herbicide resistance, insect resistance, disease resistance or acombination thereof.

In certain embodiments, methods for producing a wheat plant are providedin which plant breeding techniques are applied to a wheat plant grownfrom seed of wheat variety W030258E2 comprising a locus conversion, orto a plant grown from seed of a cross of such a wheat plant to adifferent wheat plant.

DETAILED DESCRIPTION

Field crops are bred through techniques that take advantage of theplant's method of pollination. A plant is self-pollinated if pollen fromone flower is transferred to the same or another flower of the sameplant. A plant is sib-pollinated when individuals within the same familyor line are used for pollination. A plant is cross-pollinated if thepollen comes from a flower on a different plant from a different familyor line. The term cross-pollination herein does not includeself-pollination or sib-pollination. Wheat plants (Triticum aestivumL.), are recognized to be naturally self-pollinated plants which, whilecapable of undergoing cross-pollination, rarely do so in nature. Thusintervention for control of pollination is critical to the establishmentof superior varieties.

In order to cross pollinate one wheat plant with another to produceprogeny with a new combination of genetic traits, a method ofcross-pollination is employed. Cross-pollination is known to thoseskilled in the art. Wheat cross-pollination is achieved by emasculatingflowers of a designated female plant and pollinating the female parentwith pollen from the designated male parent. The following method wasemployed to cross-pollinate the wheat plants, but other methods can beused, or modified, as is known to those skilled in the art.

The designated female wheat plant is emasculated before its anthers shedpollen to avoid self-pollination. Emasculation is done by selecting animmature spike on the designated female parent plant that has notstarted to bloom and shed any viable pollen. Each spike consists of aseries of spikelets composed of florets which each contain one ovarywith a feathery stigma and three anthers. Typically all but the twoprimary florets are removed from each spikelet by using tweezers. Theglumes of each remaining floret can be trimmed back about 50% usingscissors to expose the immature anthers. The tweezers are used to spreadthe glumes slightly open while at the same time surrounding the anthers.The anthers can then be removed by gently grabbing and pulling them outof the flower with the tweezers in an upward motion. With skill, allthree anthers can be removed at once, but this must be confirmedvisually before moving to the next flower. Repeated attempts to removeany remaining anthers increases the risk of damage to the stigma andovary, which will greatly reduce the frequency of cross-pollination.After all the florets are emasculated on a spike, it is covered with acellophane bag to prevent pollination with stray pollen from surroundingplants. One to three days after the female spike is emasculated a maturespike that is shedding pollen is selected from the designated male plantfor cross-pollination using the approach method. The stem of the malespike is cut off at least one foot below the spike and typically theglumes of all the spikelets are trimmed back with scissors to encourageanther extrusion during pollination. The stem of the male spike isplaced in a test tube full of water, which is attached to a stickimplanted beside the emasculated female spike. The male spike is placedabove the emasculated female spike(s) in the same cellophane bag and itis permitted to shed pollen naturally over the next several days. Bywaiting a few days after emasculation, one can ensure that no anthers orviable pollen has remained in the female spike and the stigmas becomemore receptive to cross-pollination. Emasculated female spikes that areeffectively cross-pollinated by the designated male parent willtypically set 10-30 seeds per spike. Depending on the breedingobjectives, one to five spikes are typically cross-pollinated for eachcross. Spikes from the cross are hand harvested and the F1 seed from thespikes are advanced to the F1 generation. The F1 plants can be used forsubsequent cross-pollination or they can be advanced to the F2generation for selection and further advancement. For the F2 grow out,2500 to 3500 seeds are typically planted.

A cross between two different homozygous lines produces a uniformpopulation of hybrid plants that may be heterozygous for many gene loci.A cross of two heterozygous plants each that differ at a number of geneloci will produce a population of plants that differ genetically andwill not be uniform. Regardless of parentage, plants that have beenself-pollinated and selected for type for many generations becomehomozygous at almost all gene loci and produce a uniform population oftrue breeding progeny. The term “homozygous plant” is hereby defined asa plant with homozygous genes at 95% or more of its loci.

Choice of breeding or selection methods depends on the mode of plantreproduction, the heritability of the trait(s) being improved, and thetype of variety used commercially (e.g., F1 hybrid variety, purelinevariety, etc.). For highly heritable traits, a choice of superiorindividual plants evaluated at a single location will be effective,whereas for traits with low heritability, selection should be based onmean values obtained from replicated evaluations of families of relatedplants. Popular selection methods commonly include pedigree selection,modified pedigree selection, mass selection, and recurrent selection.

The complexity of inheritance influences choice of the breeding method.In general breeding starts with the crossing of two genotypes (a“breeding cross”), each of which may have one or more desirablecharacteristics that is lacking in the other or which complements theother. If the two original parents do not provide all the desiredcharacteristics, other sources can be included by making more crosses.In each successive filial generation, F1→F2; F2→F3; F3→F4; F4→F5, etc.,plants are selfed to increase the homozygosity of the line. Typically ina breeding program five or more generations of selection and selfing arepracticed to obtain a homozygous plant.

Pedigree breeding is commonly used for the improvement ofself-pollinating crops. Two parents that possess favorable,complementary traits are crossed to produce an F1. An F2 population isproduced by selfing or sibbing one or several F1's. Selection of thebest individuals may begin in the F2 population; then, beginning in theF3, the best individuals in the best families are selected. Replicatedtesting of families can begin in the F4 generation to improve theeffectiveness of selection for traits with low heritability. At anadvanced stage of inbreeding (i.e., F5, F6 and F7), the best lines ormixtures of phenotypically similar lines are tested for potentialrelease as new varieties.

Backcross breeding has been used to transfer genes for simply inherited,qualitative, traits from a donor parent into a desirable homozygousvariety that is utilized as the recurrent parent. The source of thetraits to be transferred is called the donor parent. After the initialcross, individuals possessing the desired trait or traits of the donorparent are selected and then repeatedly crossed (backcrossed) to therecurrent parent. The resulting plant is expected to have the attributesof the recurrent parent (e.g., variety) plus the desirable trait ortraits transferred from the donor parent. This approach has been usedextensively for breeding disease resistant varieties.

Each wheat breeding program should include a periodic, objectiveevaluation of the efficiency of the breeding procedure. Evaluationcriteria vary depending on the goal and objectives, but should includegain from selection per year based on comparisons to an appropriatestandard, overall value of the advanced breeding lines, and number ofsuccessful varieties produced per unit of input (e.g., per year, perdollar expended, etc.).

Various recurrent selection techniques are used to improvequantitatively inherited traits controlled by numerous genes. The use ofrecurrent selection in self-pollinating crops depends on the ease ofpollination and the number of hybrid offspring from each successfulcross. Recurrent selection can be used to improve populations of eitherself- or cross-pollinated crops. A genetically variable population ofheterozygous individuals is either identified or created byintercrossing several different parents. The best plants are selectedbased on individual superiority, outstanding progeny, or excellentcombining ability. The selected plants are intercrossed to produce a newpopulation in which further cycles of selection are continued. Plantsfrom the populations can be selected and selfed to create new varieties.

Another breeding method is single-seed descent. This procedure in thestrict sense refers to planting a segregating population, harvesting asample of one seed per plant, and using the one-seed sample to plant thenext generation. When the population has been advanced from the F2 tothe desired level of inbreeding, the plants from which lines are derivedwill each trace to different F2 individuals. The number of plants in apopulation declines each generation due to failure of some seeds togerminate or some plants to produce at least one seed. As a result, notall of the F2 plants originally sampled in the population will berepresented by a progeny when generation advance is completed. In amultiple-seed procedure, wheat breeders commonly harvest one or morespikes (heads) from each plant in a population and thresh them togetherto form a bulk. Part of the bulk is used to plant the next generationand part is put in reserve. The procedure has been referred to asmodified single-seed descent. The multiple-seed procedure has been usedto save labor at harvest. It is considerably faster to thresh spikeswith a machine than to remove one seed from each by hand for thesingle-seed procedure. The multiple-seed procedure also makes itpossible to plant the same number of seeds of a population eachgeneration of inbreeding. Enough seeds are harvested to make up forthose plants that did not germinate or produce seed.

Bulk breeding can also be used. In the bulk breeding method an F2population is grown. The seed from the populations is harvested in bulkand a sample of the seed is used to make a planting the next season.This cycle can be repeated several times. In general when individualplants are expected to have a high degree of homozygosity, individualplants are selected, tested, and increased for possible use as avariety.

Molecular markers including techniques such as Starch GelElectrophoresis, Isozyme Electrophoresis, Restriction Fragment LengthPolymorphisms (RFLPs), Randomly Amplified Polymorphic DNAs (RAPDs),Arbitrarily Primed Polymerase Chain Reaction (AP-PCR), DNA AmplificationFingerprinting (DAF), Sequence Characterized Amplified Regions (SCARs),Amplified Fragment Length Polymorphisms (AFLPs), Simple Sequence Repeats(SSRs), and Single Nucleotide Polymorphisms (SNPs) may be used in plantbreeding methods. One use of molecular markers is Quantitative TraitLoci (QTL) mapping. QTL mapping is the use of markers, which are knownto be closely linked to alleles that have measurable effects on aquantitative trait. Selection in the breeding process is based upon theaccumulation of markers linked to the positive effecting alleles and/orthe elimination of the markers linked to the negative effecting allelesfrom the plant's genome.

Molecular markers can also be used during the breeding process for theselection of qualitative traits. For example, markers closely linked toalleles or markers containing sequences within the actual alleles ofinterest can be used to select plants that contain the alleles ofinterest during a backcrossing breeding program. The markers can also beused to select for the genome of the recurrent parent and against themarkers of the donor parent. Using this procedure can minimize theamount of genome from the donor parent that remains in the selectedplants. It can also be used to reduce the number of crosses back to therecurrent parent needed in a backcrossing program (Openshaw et al.Marker-assisted Selection in Backcross Breeding. In: ProceedingsSymposium of the Analysis of Molecular Marker Data, 5-6 Aug. 1994, pp.41-43. Crop Science Society of America, Corvallis, Oreg.). The use ofmolecular markers in the selection process is often called GeneticMarker Enhanced Selection.

The production of double haploids can also be used for the developmentof homozygous lines in the breeding program. Double haploids areproduced by the doubling of a set of chromosomes (1 N) from aheterozygous plant to produce a completely homozygous individual. Thiscan be advantageous because the process omits the generations of selfingneeded to obtain a homozygous plant from a heterozygous source. Variousmethodologies of making double haploid plants in wheat have beendeveloped (Laurie, D. A. and S. Reymondie, Plant Breeding, 1991, v.106:182-189. Singh, N. et al., Cereal Research Communications, 2001, v.29:289-296; Redha, A. et al., Plant Cell Tissue and Organ Culture, 2000,v. 63:167-172; U.S. Pat. No. 6,362,393)

Though pure-line varieties are the predominate form of wheat grown forcommercial wheat production hybrid wheat is also used. Hybrid wheats areproduced with the help of cytoplasmic male sterility, nuclear geneticmale sterility, or chemicals. Various combinations of these three malesterility systems have been used in the production of hybrid wheat.

Descriptions of other breeding methods that are commonly used fordifferent traits and crops can be found in one of several referencebooks (e.g., Allard, Principles of Plant Breeding, 1960; Simmonds,Principles of Crop Improvement, 1979; editor Heyne, Wheat and WheatImprovement, 1987; Allan, “Wheat”, Chapter 18, Principles of CropDevelopment, vol. 2, Fehr editor, 1987).

Promising advanced breeding lines are thoroughly tested and compared toappropriate standards in environments representative of the commercialtarget area(s). The best lines are candidates for new commercialvarieties; those still deficient in a few traits may be used as parentsto produce new populations for further selection.

A most difficult task is the identification of individuals that aregenetically superior, because for most traits the true genotypic valueis masked by other confounding plant traits or environmental factors.One method of identifying a superior genotype is to observe itsperformance relative to other experimental genotypes and to a widelygrown standard variety. Generally a single observation is inconclusive,so replicated observations are required to provide a better estimate ofits genetic worth.

A breeder uses various methods to help determine which plants should beselected from the segregating populations and ultimately which lineswill be used for commercialization. In addition to the knowledge of thegermplasm and other skills the breeder uses, a part of the selectionprocess is dependent on experimental design coupled with the use ofstatistical analysis. Experimental design and statistical analysis areused to help determine which plants, which family of plants, and finallywhich lines are significantly better or different for one or more traitsof interest. Experimental design methods are used to control error sothat differences between two lines can be more accurately determined.Statistical analysis includes the calculation of mean values,determination of the statistical significance of the sources ofvariation, and the calculation of the appropriate variance components.Five and one percent significance levels are customarily used todetermine whether a difference that occurs for a given trait is real ordue to the environment or experimental error.

Plant breeding is the genetic manipulation of plants. The goal of wheatbreeding is to develop new, unique and superior wheat varieties. Inpractical application of a wheat breeding program, the breeder initiallyselects and crosses two or more parental lines, followed by repeatedselfing and selection, producing many new genetic combinations. Thebreeder can theoretically generate billions of different geneticcombinations via crossing, selfing and mutations.

Each year, the plant breeder selects the germplasm to advance to thenext generation. This germplasm is grown under unique and differentgeographical, climatic and soil conditions, and further selections arethen made during and at the end of the growing season.

Proper testing should detect major faults and establish the level ofsuperiority or improvement over current varieties. In addition toshowing superior performance, there must be a demand for a new variety.The new variety must be compatible with industry standards, or mustcreate a new market. The introduction of a new variety may incuradditional costs to the seed producer, the grower, processor andconsumer, for special advertising and marketing, altered seed andcommercial production practices, and new product utilization. Thetesting preceding release of a new variety should take intoconsideration research and development costs as well as technicalsuperiority of the final variety. It must also be feasible to produceseed easily and economically.

These processes, which lead to the final step of marketing anddistribution, can take from six to twelve years from the time the firstcross is made. Therefore, development of new varieties is atime-consuming process that requires precise forward planning, efficientuse of resources, and a minimum of changes in direction.

Wheat (Triticum aestivum L.), is an important and valuable field crop.Thus, a continuing goal of wheat breeders is to develop stable, highyielding wheat varieties that are agronomically sound and have goodgrain quality for its intended use. To accomplish this goal, the wheatbreeder must select and develop wheat plants that have the traits thatresult in superior varieties.

According to the invention, there is provided a novel wheat variety,designated W030258E2 and processes for making W030258E2. This inventionrelates to seed of wheat variety W030258E2, to the plants of wheatvariety W030258E2, to plant parts of wheat variety W030258E2, and toprocesses for making a wheat plant that comprise crossing wheat varietyW030258E2 with another wheat plant. This invention also relates toprocesses for making a wheat plant containing in its genetic materialone or more traits introgressed into W030258E2 through backcrossconversion and/or transformation, and to the wheat seed, plant and plantparts produced thereby. This invention also relates to the creation ofvariants by mutagenesis or transformation of wheat W030258E2. Thisinvention further relates to a hybrid wheat seed, plant or plant partproduced by crossing the variety W030258E2 or a locus conversion ofW030258E2 with another wheat variety.

Wheat varieties that are highly homogeneous, homozygous and reproducibleare useful as commercial varieties. There are many analytical methodsavailable to determine the homozygotic stability, phenotypic stability,and identity of these varieties.

The oldest and most traditional method of analysis is the observation ofphenotypic traits. The data is usually collected in field experimentsover the life of the wheat plants to be examined. Phenotypiccharacteristics most often observed are for traits such as seed yield,head configuration, glume configuration, seed configuration, lodgingresistance, disease resistance, maturity, etc.

In addition to phenotypic observations, the genotype of a plant can alsobe examined. There are many laboratory-based techniques available forthe analysis, comparison and characterization of plant genotype; amongthese are Gel Electrophoresis, Isozyme Electrophoresis, RestrictionFragment Length Polymorphisms (RFLPs), Randomly Amplified PolymorphicDNAs (RAPDs), Arbitrarily Primed Polymerase Chain Reaction (AP-PCR), DNAAmplification Fingerprinting (DAF), Sequence Characterized AmplifiedRegions (SCARs), Amplified Fragment Length Polymorphisms (AFLPs), SimpleSequence Repeats (SSRs) which are also referred to as Microsatellites,Genotype by Sequence (GBS), and Single Nucleotide Polymorphisms (SNPs).Gel electrophoresis is particularly useful in wheat. Wheat varietyidentification is possible through electrophoresis of gliadin, glutenin,albumin and globulin, and total protein extracts (Bietz, J. A., pp.216-228, “Genetic and Biochemical Studies of Nonenzymatic EndospermProteins” In Wheat and Wheat Improvement, ed. E. G. Heyne, 1987).

The variety of the invention has shown uniformity and stability for alltraits, as described in the following variety description information.It has been self-pollinated a sufficient number of generations, withcareful attention to uniformity of plant type to ensure homozygosity andphenotypic stability. The line has been increased with continuedobservation for uniformity. No variant traits have been observed or areexpected in W030258E2, as described in Table 1 (Variety DescriptionInformation).

Wheat variety W030258E2 is a common, soft red winter wheat. VarietyW030258E2 demonstrates excellent yield potential, very good test weight,strong straw lodging resistance, very good resistance to leaf rust, andgood resistance to Fusarium head blight (scab). Variety W030258E2demonstrates below average powdery mildew resistance. Variety W030258E2has medium maturity relative to other varieties in the primary region ofadaptation. It has shown adaptation to the northern soft wheat regionsbased on tests conducted in Arkansas, Georgia, Illinois, Indiana,Kentucky, Michigan, Missouri, Mississippi, North Carolina, Ohio,Tennessee, Virginia, and Ontario, Canada.

Wheat variety W030258E2 was developed by from a cross between threehomozygous lines: W950586C1, W960095H1, and 25R47. First W950586C1 andW960095H1 were crossed to produce F1 seed then the F1 plants werecrossed with 25R47. Wheat variety W030258E2, being substantiallyhomozygous, can be reproduced by planting seeds of the line, growing theresulting wheat plants under self-pollinating conditions, and harvestingthe resulting seed, using techniques familiar to the agricultural arts.

The subsequent breeding history of W030258E2 is described below.

Year Generation 2002 Final cross Final cross made in Windfall, IN. 2003F1 F₁ grown in transplant nursery at Windfall, IN. 2003-04 F2 Bulkpopulations grown at Windfall, IN and Princeton, IN. Individual spikeselections made at both locations. 2004-05 F3 Headrows from F₂selections grown at Windfall, IN and Princeton, IN. Selected rows cutand threshed individually. This selection was made at Princeton, IN.2005-06 F4 A three row × 3-meter observation plot was planted atWindfall, IN and Princeton, IN. A meter section of the center row washarvested from the selected plot at Windfall, IN and threshed in bulk.2006-07 F5 A seven row × 3-meter plot was planted at Windfall, IN andPrinceton, IN. Spikes were harvested from the selected plot in Windfall,IN and threshed individually. 2007-08 F6 Twenty headrows each of the F₅selection were grown at Windfall, IN and Princeton, IN. Selected rowswere cut and threshed individually. This selection was made atPrinceton, IN. 2008-09 F7 Preliminary yield testing of an F₅ selectionfrom an F₆ headrow. This selection designated W030258E2. Individualheads were harvested from this selection. 2009-10 F8 Advanced yieldtesting of W030258E2. Eight purification headrows were planted, off-typerows destroyed prior to maturity, and 100 heads harvested from remainingrows and threshed individually. 2010-11 F9 Elite yield testing ofW030258E2. 100 purification headrows planted, off-type rows destroyedprior to maturity. Remaining rows were individually cut and threshed.2011-12 F10 Elite yield testing continued of W030258E2. Seed frompurification headrows planted in individual progeny plots. Off-typeplots were destroyed prior to harvest. The remaining progeny plots wereharvested in bulk, which constitutes Breeder Seed. 2012-13 F11Performance testing, purification and increase continued. 2013-14 F12Performance testing, purification and increase continued.

The experimental cultivar W030258E2 was bred and selected using amodified pedigree selection method for any and all of the followingcharacteristics in the field environment: disease resistance, planttype, plant height, head type, straw strength, maturity, grain yield,test weight, and milling and baking characteristics. W030258E2 has beenshown to be uniform and stable since the 7th generation, or for the last6 generations. W030258E2 has shown no variants other than what wouldnormally be expected due to environment.

Definitions for Area of Adaptability

When referring to area of adaptability, such term is used to describethe location with the environmental conditions that would be well suitedfor this wheat variety. Area of adaptability is based on a number offactors, for example: days to heading, winter hardiness, insectresistance, disease resistance, and drought resistance. Area ofadaptability does not indicate that the wheat variety will grow in everylocation within the area of adaptability or that it will not growoutside the area.

Northern area=States of DE, IL, IN, MI, MO, NJ, NY, OH, PA, WI andOntario, Canada

Mid-south=States of AR, KY, MO bootheel and TN

Southeast=States of NC, SC, and VA

Deep South=States of AL, GA, LA, and MS

TABLE 1 VARIETY DESCRIPTION INFORMATION W030258E2 1. KIND: 1 (1 =Common, 2 = Durum, 3 = Club, 4 = Other) 2. VERNALIZATION: 2 (1 = Spring,2 = Winter, 3 = Other) 3. COLEOPTILE ANTHOCYANIN: 1(1 = Absent, 2 =Present) 4. JUVENILE PLANT GROWTH: 2 (1 = Prostrate, 2 = Semi-erect, 3 =Erect) 5. PLANT COLOR (boot stage): 2 (1 = Yellow-Green, 2 = Green, 3 =Blue-Green) 6. FLAG LEAF (boot stage): 1 (1 = Erect, 2 = Recurved) FLAGLEAF (boot stage): 2 (1 = Not Twisted, 2 = Twisted) FLAG LEAF (bootstage): 2 (1 = Wax Absent, 2 = Wax Present) 7. EAR EMERGENCE: 124 =Number of Days after Jan. 1 and 1 Day later than 25R47 8. ANTHER COLOR:2 (1 = Yellow, 2 = Purple) 9. PLANT HEIGHT (from soil to top of head,excluding awns): 81 cm (Average) 2.5 cm taller than 25R47 10. STEM: A.ANTHOCYANIN: 2 (1 = Absent, 2 = Present) B. WAXY BLOOM: 2 (1 = Absent, 2= Present) C. HAIRINESS (last internode of rachis): 2 (1 = Absent, 2 =Present) D. INTERNODE: 1 (1 = Hollow, 2 = Semi-solid, 3 = Solid) E.PEDUNCLE: 3 (1 = Erect, 2 = Recurved, 3 = Semi-erect) F. AURICLEAnthocyanin: 2 (1 = Absent, 2 = Present) Hair: 1 (1 = Absent, 2 =Present) 11. HEAD (at maturity) A. DENSITY: 2 (1 = Lax, 2 = Middense, 3= Dense) B. SHAPE: 2(1 = Tapering, 2 = Strap, 3 = Clavate, 4 = Other) C.CURVATURE: 2 (1 = Erect, 2 = Inclined, 3 = Recurved) D. AWNEDNESS: 4 (1= Awnless, 2 = Apically Awnletted, 3 = Awnletted, 4 = Awned) 12. GLUMES(at Maturity): A. COLOR: 2 (1 = White, 2 = Tan, 3 = Other) B. SHOULDER:2 (1 = Wanting, 2 = Oblique, 3 = Rounded, 4 = Square, 5 = Elevated, 6 =Apiculate) C. SHOULDER WIDTH: 2 (1 = Narrow, 2 = Medium, 3 = Wide) D.BEAK: 3 (1 = Obtuse, 2 = Acute, 3 = Acuminate) E. BEAK WIDTH: 2 (1 =Narrow, 2 = Medium, 3 = Wide) F. GLUME LENGTH: 1 (1 = Short (ca. 7 mm),2 = Medium (ca. 8 mm), 3 = Long (ca. 9 mm)) G. GLUME WIDTH: 3 (1 =Narrow (ca. 3 mm), 2 = Medium (ca. 3.5 mm), 3 = Wide (ca. 4 mm) H.PUBESCENCE: 1 (1 = Not Present 2 = Present) 13. SEED: A. SHAPE: 2 (1 =Ovate, 2 = Oval, 3 = Elliptical) B. CHEEK: 1 (1 = Rounded, 2 = Angular)C. BRUSH: 2 (1 = Short, 2 = Medium, 3 = Long) BRUSH: 1 (1 = NotCollared, 2 = Collared) D. CREASE: 1 (1 = Width 60% or less of Kernel, 2= Width 80% or less of Kernel, 3 = Width Nearly as Wide as Kernel)CREASE: 1 (1 = Depth 20% or less of Kernel, 2 = Depth 35%, or less ofKernel, 3 = Depth 50% or less of Kernel) E. COLOR: 3 (1 = White, 2 =Amber, 3 = Red, 4 = Other) F. TEXTURE: 2 (1 = Hard, 2 = Soft, 3 = Other)G. PHENOL REACTION: 3 (1 = Ivory, 2 = Fawn, 3 = Light Brown, 4 = DarkBrown 5 = Black) H. SEED WEIGHT: 40 g/1000 Seed I. GERM SIZE: 2 (1 =Small, 2 = Midsize, 3 = Large) 14. DISEASE: (0 = Not tested, 1 =Susceptible, 2 = Resistant, 3 = Intermediate, 4 = Tolerant) SPECIFICRACE OR STRAIN TESTED Stem Rust (Puccinia graminis f. sp. tritici) 0Leaf Rust (Puccinia recondita f. sp. tritici) 3 Stripe Rust (Pucciniastriiformis) 3 Loose Smut (Ustilago tritici) 0 Powdery Mildew (erysiphegraminis f.sp.tritici) 1 Common Bunt (Tilletia tritici or T. laevis) 0Dwarf Bunt (Tilletia controversa) 0 Karnal Bunt (Tilletia indica) 0 FlagSmut (Urocystis agropyri) 0 Tan Spot (Pyrenophora tritici-repentis) 0Halo Spot (Selenophoma donacis) 0 Septoria spp. 0 Septoria nodorum(Glume Blotch) 0 Septoria avenae (Speckled Leaf Disease) 0 Septoriatritici (Speckled Leaf Blotch) 3 Scab (Fusarium spp.) 3 “Snow Molds” 0Kernel Smudge (“Black Point”) 0 Common Root Rot (Fusarium, Cochliobolus,& Bipolaris spp.) 0 Barley Yellow Dwarf Virus (BYDV) 0 Rhizoctonia RootRot (Rhizoctonia solani) 0 Soilborne Mosaic Virus (SBMV) 1 Black Chaff(Xanthomonas campestris pv. translucens) 0 Wheat Yellow (Spindle Streak)Mosaic Virus 0 Bacterial Leaf Blight (pseudomonas syringae pv. syringae)0 Wheat Streak Mosaic Virus (WSMV) 0 15. INSECT: (0 = Not tested, 1 =Susceptible, 2 = Resistant, 3 = Intermediate, 4 = Tolerant) Stem Sawfly(Cephus spp) 0 Cereal Leaf Beetle (Oulema melanopa) 0 Russian Aphid(Diuraphis noxia) 0 Greenbug (schizaphis graminum) (General) 0 Aphids 0Hessian Fly (Mayetiola destructor) Biotype L 1 Hessian Fly (Mayetioladestructor) (Specify) Field 1

In one aspect, wheat plants, plant parts and seeds are provided whichhave all or essentially all of the characteristics set forth in table 1.In one aspect wheat plants, plant parts and seeds are provided whichhave all or essentially all of the physiological and morphologicalcharacteristics of wheat variety W030258E2, representative seed havingbeen deposited with the ATCC as disclosed herein.

Further Embodiments of the Invention

Further reproduction of the wheat variety W030258E2 can occur by tissueculture and regeneration. Tissue culture of various tissues of wheat andregeneration of plants therefrom is well known and widely published. Areview of various wheat tissue culture protocols can be found in “InVitro Culture of Wheat and Genetic Transformation-Retrospect andProspect” by Maheshwari et al. (Critical Reviews in Plant Sciences,14(2): pp 149-178, 1995). Thus, another aspect of this invention is toprovide cells which upon growth and differentiation produce wheat plantscapable of having the physiological and morphological characteristics ofwheat variety W030258E2.

As used herein, the term plant parts includes plant protoplasts, plantcell tissue cultures from which wheat plants can be regenerated, plantcalli, plant clumps, and plant cells that are intact in plants or partsof plants, such as embryos, pollen, ovules, pericarp, seed, flowers,florets, heads, spikes, leaves, roots, root tips, anthers, and the like.The term “plant” includes plant parts. When indicating that a plant iscrossed or selfed this indicates that any plant part of the plant can beused. For instance the plant part does not need to be attached to theplant during the crossing or selfing, only the pollen might be used.

The advent of new molecular biological techniques has allowed theisolation and characterization of genetic elements with specificfunctions, such as encoding specific protein products. Scientists in thefield of plant biology developed a strong interest in engineering thegenome of plants to contain and express foreign genetic elements, oradditional, or modified versions of native or endogenous geneticelements in order to alter the traits of a plant in a specific manner.Any DNA sequences, whether from a different species or from the samespecies that are inserted into the genome using transformation arereferred to herein collectively as “transgenes”. Over the last fifteento twenty years several methods for producing transgenic plants havebeen developed, and the present invention, in particular embodiments,also relates to transformed versions of the wheat variety W030258E2.

Numerous methods for plant transformation have been developed, includingbiological and physical, plant transformation protocols. See, forexample, Miki et al., “Procedures for Introducing Foreign DNA intoPlants” in Methods in Plant Molecular Biology and Biotechnology, Glick,B. R. and Thompson, J. E. Eds. (CRC Press, Inc., Boca Raton, 1993) pages67-88. In addition, expression vectors and in vitro culture methods forplant cell or tissue transformation and regeneration of plants areavailable. See, for example, Gruber et al., “Vectors for PlantTransformation” in Methods in Plant Molecular Biology and Biotechnology,Glick, B. R. and Thompson, J. E. Eds. (CRC Press, Inc., Boca Raton,1993) pages 89-119.

The most prevalent types of plant transformation involve theconstruction of an expression vector. Such a vector comprises a DNAsequence that contains a gene under the control of or operatively linkedto a regulatory element, for example a promoter. The vector may containone or more genes and one or more regulatory elements.

Various genetic elements can be introduced into the plant genome usingtransformation. These elements include but are not limited to genes;coding sequences; inducible, constitutive, and tissue specificpromoters; enhancing sequences; and signal and targeting sequences.

A genetic trait which has been engineered into a particular wheat plantusing transformation techniques, could be moved into another line usingtraditional breeding techniques that are well known in the plantbreeding arts. For example, a backcrossing approach could be used tomove a transgene from a transformed wheat plant to an elite wheatvariety and the resulting progeny would comprise a transgene. As usedherein, “crossing” can refer to a simple X by Y cross, or the process ofbackcrossing, depending on the context. The term “breeding cross”excludes the processes of selfing or sibbing.

With transgenic plants according to the present invention, a foreignprotein can be produced in commercial quantities. Thus, techniques forthe selection and propagation of transformed plants, which are wellunderstood in the art, yield a plurality of transgenic plants which areharvested in a conventional manner, and a foreign protein then can beextracted from a tissue of interest or from total biomass. Proteinextraction from plant biomass can be accomplished by known methods whichare discussed, for example, by Heney and Orr, Anal. Biochem. 114: 92-6(1981).

According to a preferred embodiment, the transgenic plant provided forcommercial production of foreign protein is a wheat plant. In anotherpreferred embodiment, the biomass of interest is seed. A genetic map canbe generated, primarily via conventional RFLP, PCR, and SSR analysis,which identifies the approximate chromosomal location of the integratedDNA molecule. For exemplary methodologies in this regard, see Glick andThompson, METHODS IN PLANT MOLECULAR BIOLOGY AND BIOTECHNOLOGY 269-284(CRC Press, Boca Raton, 1993). Map information concerning chromosomallocation is useful for proprietary protection of a subject transgenicplant. If unauthorized propagation is undertaken and crosses made withother germplasm, the map of the integration region can be compared tosimilar maps for suspect plants, to determine if the latter have acommon parentage with the subject plant. Map comparisons would involvehybridizations, RFLP, PCR, SSR, SNPS and sequencing, all of which areconventional techniques.

Likewise, by means of the present invention, agronomic genes can beexpressed in transformed plants. More particularly, plants can begenetically engineered to express various phenotypes of agronomicinterest. Through the transformation of wheat the expression of genescan be modulated to enhance disease resistance, insect resistance,herbicide resistance, water stress tolerance and agronomic traits aswell as grain quality traits. Transformation can also be used to insertDNA sequences which control or help control male-sterility. DNAsequences native to wheat as well as non-native DNA sequences can betransformed into wheat and used to modulate levels of native ornon-native proteins. Anti-sense technology, various promoters, targetingsequences, enhancing sequences, and other DNA sequences can be insertedinto the wheat genome for the purpose of modulating the expression ofproteins. Exemplary genes implicated in this regard include, but are notlimited to, those categorized below.

1. Genes that Confer Resistance to Pests or Disease and that Encode:

(A) Plant disease resistance genes. Plant defenses are often activatedby specific interaction between the product of a disease resistance gene(R) in the plant and the product of a corresponding avirulence (Avr)gene in the pathogen. A plant variety can be transformed with clonedresistance gene to engineer plants that are resistant to specificpathogen strains. See, for example Jones et al., Science 266: 789 (1994)(cloning of the tomato Cf-9 gene for resistance to Cladosporium fulvum);Martin et al., Science 262: 1432 (1993) (tomato Pto gene for resistanceto Pseudomonas syringae pv. tomato encodes a protein kinase); Mindrinoset al., Cell 78: 1089 (1994) (Arabidopsis RSP2 gene for resistance toPseudomonas syringae); McDowell & Woffenden, (2003) Trends Biotechnol.21(4): 178-83 and Toyoda et al., (2002) Transgenic Res. 11(6):567-82. Aplant resistant to a disease is one that is more resistant to a pathogenas compared to the wild type plant.

Fusarium head blight along with deoxynivalenol both produced by thepathogen Fusarium graminearum Schwabe have caused devastating losses inwheat production. Genes expressing proteins with antifungal action canbe used as transgenes to prevent Fusarium head blight. Various classesof proteins have been identified. Examples include endochitinases,exochitinases, glucanases, thionins, thaumatin-like proteins, osmotins,ribosome inactivating proteins, flavoniods, lactoferricin. Duringinfection with Fusarium graminearum deoxynivalenol is produced. There isevidence that production of deoxynivalenol increases the virulence ofthe disease. Genes with properties for detoxification of deoxynivalenol(Adam and Lemmens, In International Congress on Molecular Plant-MicrobeInteractions, 1996; McCormick et al. Appl. Environ. Micro. 65:5252-5256,1999) have been engineered for use in wheat. A synthetic peptide thatcompetes with deoxynivalenol has been identified (Yuan et al., Appl.Environ. Micro. 65:3279-3286, 1999). Changing the ribosomes of the hostso that they have reduced affinity for deoxynivalenol has also been usedto reduce the virulence of the Fusarium graminearum.

Genes used to help reduce Fusarium head blight include but are notlimited to Tri101 (Fusarium), PDR5 (yeast), tlp-1 (oat), tlp-2(oat),leaf tlp-1 (wheat), tlp (rice), tlp-4 (oat), endochitinase,exochitinase, glucanase (Fusarium), permatin (oat), seed hordothionin(barley), alpha-thionin (wheat), acid glucanase (alfalfa), chitinase(barley and rice), class beta II-1,3-glucanase (barley), PR5/tlp(arabidopsis), zeamatin (maize), type 1 RIP (barley), NPR1(arabidopsis), lactoferrin (mammal), oxalyl-CoA-decarboxylase(bacterium), IAP (baculovirus), ced-9 (C. elegans), and glucanase (riceand barley).

(B) A gene conferring resistance to a pest, such as Hessian fly, wheat,stemsawfly, cereal leaf beetle, and/or green bug. For example the H9,H10, and H21 genes for resistance to Hessian fly.

(C) A gene conferring resistance to disease, including wheat rusts,Septoria tritici, Septoria nodorum, powdery mildew, Helminthosporiumdiseases, smuts, bunts, Fusarium diseases, bacterial diseases, and viraldiseases.

(D) A Bacillus thuringiensis protein, a derivative thereof or asynthetic polypeptide modeled thereon. See, for example, Geiser et al.,Gene 48: 109 (1986), who disclose the cloning and nucleotide sequence ofa Bt delta-endotoxin gene. Moreover, DNA molecules encodingdelta-endotoxin genes can be purchased from American Type CultureCollection (Rockville, Md.), for example, under ATCC Accession Nos.40098, 67136, 31995 and 31998. Other examples of Bacillus thuringiensistransgenes encoding a endotoxin and being genetically engineered aregiven in the following patents and patent applications and hereby areincorporated by reference for this purpose: U.S. Pat. Nos. 5,188,960;5,689,052; 5,880,275; WO 91/14778; WO 99/31248; WO 01/12731; WO99/24581; WO 97/40162 and U.S. application Ser. Nos. 10/032,717;10/414,637; and 10/606,320.

(E) An insect-specific hormone or pheromone such as an ecdysteroid andjuvenile hormone, a variant thereof, a mimetic based thereon, or anantagonist or agonist thereof. See, for example, the disclosure byHammock et al., Nature 344: 458 (1990), of baculovirus expression ofcloned juvenile hormone esterase, an inactivator of juvenile hormone.

(F) An insect-specific peptide which, upon expression, disrupts thephysiology of the affected pest. For example, see the disclosures ofRegan, J. Biol. Chem. 269: 9 (1994) (expression cloning yields DNAcoding for insect diuretic hormone receptor); Pratt et al., Biochem.Biophys. Res. Comm. 163: 1243 (1989) (an allostatin is identified inDiploptera puntata); Chattopadhyay et al. (2004) Critical Reviews inMicrobiology 30 (1): 33-54 2004; Zjawiony (2004) J Nat Prod 67 (2):300-310; Carlini & Grossi-de-Sa (2002) Toxicon, 40 (11): 1515-1539;Ussuf et al. (2001) Curr Sci. 80 (7): 847-853; and Vasconcelos &Oliveira (2004) Toxicon 44 (4): 385-403. See also U.S. Pat. No.5,266,317 to Tomalski et al., who disclose genes encodinginsect-specific toxins.

(G) An enzyme responsible for an hyperaccumulation of a monterpene, asesquiterpene, a steroid, hydroxamic acid, a phenylpropanoid derivativeor another non-protein molecule with insecticidal activity.

(H) An enzyme involved in the modification, including thepost-translational modification, of a biologically active molecule; forexample, a glycolytic enzyme, a proteolytic enzyme, a lipolytic enzyme,a nuclease, a cyclase, a transaminase, an esterase, a hydrolase, aphosphatase, a kinase, a phosphorylase, a polymerase, an elastase, achitinase and a glucanase, whether natural or synthetic. See PCTapplication WO 93/02197 in the name of Scott et al., which discloses thenucleotide sequence of a callase gene. DNA molecules which containchitinase-encoding sequences can be obtained, for example, from the ATCCunder Accession Nos. 39637 and 67152. See also Kramer et al., InsectBiochem. Molec. Biol. 23: 691 (1993), who teach the nucleotide sequenceof a cDNA encoding tobacco hookworm chitinase, and Kawalleck et al.,Plant Molec. Biol. 21: 673 (1993), who provide the nucleotide sequenceof the parsley ubi4-2 polyubiquitin gene, U.S. application Ser. Nos.10/389,432, 10/692,367, and U.S. Pat. No. 6,563,020.

(I) A molecule that stimulates signal transduction. For example, see thedisclosure by Botella et al., Plant Molec. Biol. 24: 757 (1994), ofnucleotide sequences for mung bean calmodulin cDNA clones, and Griess etal., Plant Physiol. 104: 1467 (1994), who provide the nucleotidesequence of a maize calmodulin cDNA clone.

(J) A hydrophobic peptide. See PCT application WO 95/16776 and U.S. Pat.No. 5,580,852 (disclosure of peptide derivatives of Tachyplesin whichinhibit fungal plant pathogens) and PCT application WO 95/18855 and U.S.Pat. No. 5,607,914) (teaches synthetic antimicrobial peptides thatconfer disease resistance).

(K) A membrane permease, a channel former or a channel blocker. Forexample, see the disclosure by Jaynes et al., Plant Sci. 89: 43 (1993),of heterologous expression of a cecropin-beta lytic peptide analog torender transgenic tobacco plants resistant to Pseudomonas solanacearum.

(L) A viral-invasive protein or a complex toxin derived therefrom. Forexample, the accumulation of viral coat proteins in transformed plantcells imparts resistance to viral infection and/or disease developmenteffected by the virus from which the coat protein gene is derived, aswell as by related viruses. See Beachy et al., Ann. Rev. Phytopathol.28: 451 (1990). Coat protein-mediated resistance has been conferred upontransformed plants against alfalfa mosaic virus, cucumber mosaic virus,tobacco streak virus, potato virus X, potato virus Y, tobacco etchvirus, tobacco rattle virus and tobacco mosaic virus. Id.

(M) An insect-specific antibody or an immunotoxin derived therefrom.Thus, an antibody targeted to a critical metabolic function in theinsect gut would inactivate an affected enzyme, killing the insect. Cf.Taylor et al., Abstract #497, SEVENTH INT'L SYMPOSIUM ON MOLECULARPLANT-MICROBE INTERACTIONS (Edinburgh, Scotland, 1994) (enzymaticinactivation in transgenic tobacco via production of single-chainantibody fragments).

(N) A virus-specific antibody. See, for example, Tavladoraki et al.,Nature 366: 469 (1993), who show that transgenic plants expressingrecombinant antibody genes are protected from virus attack.

(O) A developmental-arrestive protein produced in nature by a pathogenor a parasite. Thus, fungal endo alpha-1,4-D-polygalacturonasesfacilitate fungal colonization and plant nutrient release bysolubilizing plant cell wall homo-alpha-1,4-D-galacturonase. See Lamb etal., Bio/Technology 10: 1436 (1992). The cloning and characterization ofa gene which encodes a bean endopolygalacturonase-inhibiting protein isdescribed by Toubart et al., Plant J. 2: 367 (1992).

(P) A developmental-arrestive protein produced in nature by a plant. Forexample, Logemann et al., Bio/Technology 10: 305 (1992), have shown thattransgenic plants expressing the barley ribosome-inactivating gene havean increased resistance to fungal disease.

(Q) Genes involved in the Systemic Acquired Resistance (SAR) Responseand/or the pathogenesis related genes. Briggs, S., Current Biology,5(2):128-131 (1995), Pieterse & Van Loon (2004) Curr. Opin. Plant Bio.7(4):456-64 and Somssich (2003) Cell 113(7):815-6.

(R) Antifungal genes (Cornelissen and Melchers, Pl. Physiol.101:709-712, (1993) and Parijs et al., Planta 183:258-264, (1991) andBushnell et al., Can. J. of Plant Path. 20(2):137-149 (1998). Also seeU.S. application Ser. No. 09/950,933.

(S) Detoxification genes, such as for fumonisin, beauvericin,moniliformin and zearalenone and their structurally related derivatives.For example, see U.S. Pat. No. 5,792,931.

(T) Cystatin and cysteine proteinase inhibitors. See U.S. applicationSer. No. 10/947,979.

(U) Defensin genes. See WO03000863 and U.S. application Ser. No.10/178,213.

(V) Genes conferring resistance to nematodes. See WO 03/033651 and Urwinet. al., Planta 204:472-479 (1998), Williamson (1999) Curr Opin PlantBio. 2(4):327-31.

2. Genes that Confer Resistance to a Herbicide, for Example:

(A) Acetohydroxy acid synthase, which has been found to make plants thatexpress this enzyme resistant to multiple types of herbicides, has beenintroduced into a variety of plants (see, e.g., Hattori et al. (1995)Mol Gen Genet 246:419). Other genes that confer tolerance to herbicidesinclude: a gene encoding a chimeric protein of rat cytochrome P4507A1and yeast NADPH-cytochrome P450 oxidoreductase (Shiota et al. (1994)Plant Physiol 106:17), genes for glutathione reductase and superoxidedismutase (Aono et al. (1995) Plant Cell Physiol 36:1687, and genes forvarious phosphotransferases (Datta et al. (1992) Plant Mol Biol 20:619).

(B) A herbicide that inhibits the growing point or meristem, such as animidazalinone or a sulfonylurea. Exemplary genes in this category codefor mutant ALS and AHAS enzyme as described, for example, by Lee et al.,EMBO J. 7: 1241 (1988), and Miki et al., Theor. Appl. Genet. 80: 449(1990), respectively. See also, U.S. Pat. Nos. 5,605,011; 5,013,659;5,141,870; 5,767,361; 5,731,180; 5,304,732; 4,761,373; 5,331,107;5,928,937; and 5,378,824; and international publication WO 96/33270,which are incorporated herein by reference for this purpose.

(C) Glyphosate (resistance imparted by mutant5-enolpyruvl-3-phosphikimate synthase (EPSP) and aroA genes,respectively) and other phosphono compounds such as glufosinate(phosphinothricin acetyl transferase (PAT) and Streptomyceshygroscopicus phosphinothricin acetyl transferase (bar) genes), andpyridinoxy or phenoxy proprionic acids and cycloshexones (ACCaseinhibitor-encoding genes). See, for example, U.S. Pat. No. 4,940,835 toShah et al., which discloses the nucleotide sequence of a form of EPSPSwhich can confer glyphosate resistance. U.S. Pat. No. 5,627,061 to Barryet al. also describes genes encoding EPSPS enzymes. See also U.S. Pat.Nos. 6,566,587; 6,338,961; 6,248,876 B1; 6,040,497; 5,804,425;5,633,435; 5,145,783; 4,971,908; 5,312,910; 5,188,642; 4,940,835;5,866,775; 6,225,114 B1; 6,130,366; 5,310,667; 4,535,060; 4,769,061;5,633,448; 5,510,471; Re. 36,449; RE 37,287 E; and 5,491,288; andinternational publications EP1173580; WO 01/66704; EP1173581 andEP1173582, which are incorporated herein by reference for this purpose.Glyphosate resistance is also imparted to plants that express a genethat encodes a glyphosate oxido-reductase enzyme as described more fullyin U.S. Pat. Nos. 5,776,760 and 5,463,175, which are incorporated hereinby reference for this purpose. In addition glyphosate resistance can beimparted to plants by the over expression of genes encoding glyphosateN-acetyltransferase. See, for example, U.S. Application Serial No.US01/46227; Ser. Nos. 10/427,692 and 10/427,692. A DNA molecule encodinga mutant aroA gene can be obtained under ATCC accession No. 39256, andthe nucleotide sequence of the mutant gene is disclosed in U.S. Pat. No.4,769,061 to Comai. European Patent Application No. 0 333 033 to Kumadaet al. and U.S. Pat. No. 4,975,374 to Goodman et al. disclose nucleotidesequences of glutamine synthetase genes which confer resistance toherbicides such as L-phosphinothricin. The nucleotide sequence of aphosphinothricin-acetyl-transferase gene is provided in European PatentNo. 0 242 246 and 0 242 236 to Leemans et al. De Greef et al.,Bio/Technology 7: 61 (1989), describe the production of transgenicplants that express chimeric bar genes coding for phosphinothricinacetyl transferase activity. See also, U.S. Pat. Nos. 5,969,213;5,489,520; 5,550,318; 5,874,265; 5,919,675; 5,561,236; 5,648,477;5,646,024; 6,177,616 B1; and 5,879,903, which are incorporated herein byreference for this purpose. Exemplary genes conferring resistance tophenoxy proprionic acids and cycloshexones, such as sethoxydim andhaloxyfop, are the Acc1-S1, Acc1-S2 and Acc1-S3 genes described byMarshall et al., Theor. Appl. Genet. 83: 435 (1992).

(D) A herbicide that inhibits photosynthesis, such as a triazine (psbAand gs+ genes) and a benzonitrile (nitrilase gene). Przibilla et al.,Plant Cell 3: 169 (1991), describe the transformation of Chlamydomonaswith plasmids encoding mutant psbA genes. Nucleotide sequences fornitrilase genes are disclosed in U.S. Pat. No. 4,810,648 to Stalker, andDNA molecules containing these genes are available under ATCC AccessionNos. 53435, 67441 and 67442. Cloning and expression of DNA coding for aglutathione S-transferase is described by Hayes et al., Biochem. J. 285:173 (1992).

(E) Protoporphyrinogen oxidase (protox) is necessary for the productionof chlorophyll, which is necessary for all plant survival. The protoxenzyme serves as the target for a variety of herbicidal compounds. Theseherbicides also inhibit growth of all the different species of plantspresent, causing their total destruction. The development of plantscontaining altered protox activity which are resistant to theseherbicides are described in U.S. Pat. Nos. 6,288,306 B1; 6,282,837 B1;and 5,767,373; and international publication WO 01/12825.

3. Genes that Confer or Improve Grain Quality, Such as:

(A) Altered fatty acids, for example, by

-   -   (1) Down-regulation of stearoyl-ACP desaturase to increase        stearic acid content of the plant. See Knultzon et al., Proc.        Natl. Acad. Sci. USA 89: 2624 (1992) and WO99/64579 (Genes for        Desaturases to Alter Lipid Profiles in Corn),    -   (2) Elevating oleic acid via FAD-2 gene modification and/or        decreasing linolenic acid via FAD-3 gene modification (see U.S.        Pat. Nos. 6,063,947; 6,323,392; 6,372,965 and WO 93/11245),    -   (3) Altering conjugated linolenic or linoleic acid content, such        as in WO 01/12800,    -   (4) Altering LEC1, AGP, Dek1, Superal1, mi1ps, various Ipa genes        such as Ipa1, Ipa3, hpt or hggt. For example, see WO 02/42424,        WO 98/22604, WO 03/011015, U.S. Pat. No. 6,423,886, U.S. Pat.        No. 6,197,561, U.S. Pat. No. 6,825,397, US2003/0079247,        US2003/0204870, WO02/057439, WO03/011015 and Rivera-Madrid, R.        et. al. Proc. Natl. Acad. Sci. 92:5620-5624 (1995).

(B) Altered phosphorus content, for example, by the

-   -   (1) Introduction of a phytase-encoding gene would enhance        breakdown of phytate, adding more free phosphate to the        transformed plant. For example, see Van Hartingsveldt et al.,        Gene 127: 87 (1993), for a disclosure of the nucleotide sequence        of an Aspergillus niger phytase gene.    -   (2) Up-regulation of a gene that reduces phytate content. In        maize, this, for example, could be accomplished, by cloning and        then re-introducing DNA associated with one or more of the        alleles, such as the LPA alleles, identified in maize mutants        characterized by low levels of phytic acid, such as in Raboy et        al., Maydica 35: 383 (1990) and/or by altering inositol kinase        activity as in WO 02/059324, US2003/0009011, WO 03/027243,        US2003/0079247, WO 99/05298, U.S. Pat. No. 6,197,561, U.S. Pat.        No. 6,291,224, U.S. Pat. No. 6,391,348, WO2002/059324,        US2003/0079247, Wo98/45448, WO99/55882, WO01/04147.

(C) Altered carbohydrates effected, for example, by altering a gene foran enzyme that affects the branching pattern of starch or a genealtering thioredoxin (See U.S. Pat. No. 6,531,648). See Shiroza et al.,J. Bacteriol. 170: 810 (1988) (nucleotide sequence of Streptococcusmutans fructosyltransferase gene), Steinmetz et al., Mol. Gen. Genet.200: 220 (1985) (nucleotide sequence of Bacillus subtilis levansucrasegene), Pen et al., Bio/Technology 10: 292 (1992) (production oftransgenic plants that express Bacillus licheniformis alpha-amylase),Elliot et al., Plant Mol. Biol. 21: 515 (1993) (nucleotide sequences oftomato invertase genes), Søgaard et al., J. Biol. Chem. 268: 22480(1993) (site-directed mutagenesis of barley alpha-amylase gene), andFisher et al., Plant Physiol. 102: 1045 (1993) (maize endosperm starchbranching enzyme II), WO 99/10498 (improved digestibility and/or starchextraction through modification of UDP-D-xylose 4-epimerase, Fragile 1and 2, Ref1, HCHL, C4H), U.S. Pat. No. 6,232,529 (method of producinghigh oil seed by modification of starch levels (AGP)). The fatty acidmodification genes mentioned above may also be used to affect starchcontent and/or composition through the interrelationship of the starchand oil pathways.

(D) Altered antioxidant content or composition, such as alteration oftocopherol or tocotrienols. For example, see U.S. Pat. No. 6,787,683,US2004/0034886 and WO 00/68393 involving the manipulation of antioxidantlevels through alteration of a phytl prenyl transferase (ppt), WO03/082899 through alteration of a homogentisate geranyl geranyltransferase (hggt).

(E) Altered essential seed amino acids. For example, see U.S. Pat. No.6,127,600 (method of increasing accumulation of essential amino acids inseeds), U.S. Pat. No. 6,080,913 (binary methods of increasingaccumulation of essential amino acids in seeds), U.S. Pat. No. 5,990,389(high lysine), WO99/40209 (alteration of amino acid compositions inseeds), WO99/29882 (methods for altering amino acid content ofproteins), U.S. Pat. No. 5,850,016 (alteration of amino acidcompositions in seeds), WO98/20133 (proteins with enhanced levels ofessential amino acids), U.S. Pat. No. 5,885,802 (high methionine), U.S.Pat. No. 5,885,801 (high threonine), U.S. Pat. No. 6,664,445 (plantamino acid biosynthetic enzymes), U.S. Pat. No. 6,459,019 (increasedlysine and threonine), U.S. Pat. No. 6,441,274 (plant tryptophansynthase beta subunit), U.S. Pat. No. 6,346,403 (methionine metabolicenzymes), U.S. Pat. No. 5,939,599 (high sulfur), U.S. Pat. No. 5,912,414(increased methionine), WO98/56935 (plant amino acid biosyntheticenzymes), WO98/45458 (engineered seed protein having higher percentageof essential amino acids), WO98/42831 (increased lysine), U.S. Pat. No.5,633,436 (increasing sulfur amino acid content), U.S. Pat. No.5,559,223 (synthetic storage proteins with defined structure containingprogrammable levels of essential amino acids for improvement of thenutritional value of plants), WO96/01905 (increased threonine),WO95/15392 (increased lysine), US2003/0163838, US2003/0150014,US2004/0068767, U.S. Pat. No. 6,803,498, WO01/79516, and WO00/09706 (CesA: cellulose synthase), U.S. Pat. No. 6,194,638 (hemicellulose), U.S.Pat. No. 6,399,859 and US2004/0025203 (UDPGdH), U.S. Pat. No. 6,194,638(RGP).

4. Genes that Control Male-Sterility

There are several methods of conferring genetic male sterilityavailable, such as multiple mutant genes at separate locations withinthe genome that confer male sterility, as disclosed in U.S. Pat. Nos.4,654,465 and 4,727,219 to Brar et al. and chromosomal translocations asdescribed by Patterson in U.S. Pat. Nos. 3,861,709 and 3,710,511. Inaddition to these methods, Albertsen et al., U.S. Pat. No. 5,432,068,describe a system of nuclear male sterility which includes: identifyinga gene which is critical to male fertility; silencing this native genewhich is critical to male fertility; removing the native promoter fromthe essential male fertility gene and replacing it with an induciblepromoter; inserting this genetically engineered gene back into theplant; and thus creating a plant that is male sterile because theinducible promoter is not “on” resulting in the male fertility gene notbeing transcribed. Fertility is restored by inducing, or turning “on”,the promoter, which in turn allows the gene that confers male fertilityto be transcribed.

(A) Introduction of a deacetylase gene under the control of atapetum-specific promoter and with the application of the chemicalN-Ac-PPT (WO 01/29237).

(B) Introduction of various stamen-specific promoters (WO 92/13956, WO92/13957).

(C) Introduction of the barnase and the barstar gene (Paul et al. PlantMol. Biol. 19:611-622, 1992).

For additional examples of nuclear male and female sterility systems andgenes, see also, U.S. Pat. No. 5,859,341; U.S. Pat. No. 6,297,426; U.S.Pat. No. 5,478,369; U.S. Pat. No. 5,824,524; U.S. Pat. No. 5,850,014;and U.S. Pat. No. 6,265,640; all of which are hereby incorporated byreference.

5. Genes that create a site for site specific DNA integration. Thisincludes the introduction of FRT sites that may be used in the FLP/FRTsystem and/or Lox sites that may be used in the Cre/Loxp system. Forexample, see Lyznik, et al., Site-Specific Recombination for GeneticEngineering in Plants, Plant Cell Rep (2003) 21:925-932 and WO 99/25821,which are hereby incorporated by reference. Other systems that may beused include the Gin recombinase of phage Mu (Maeser et al., 1991; VickiChandler, The Maize Handbook ch. 118 (Springer-Verlag 1994), the Pinrecombinase of E. coli (Enomoto et al., 1983), and the R/RS system ofthe pSR1 plasmid (Araki et al., 1992).6. Genes that affect abiotic stress resistance (including but notlimited to flowering, ear and seed development, enhancement of nitrogenutilization efficiency, altered nitrogen responsiveness, droughtresistance or tolerance, cold resistance or tolerance, and saltresistance or tolerance) and increased yield under stress. For example,see: WO 00/73475 where water use efficiency is altered throughalteration of malate; U.S. Pat. No. 5,892,009, U.S. Pat. No. 5,965,705,U.S. Pat. No. 5,929,305, U.S. Pat. No. 5,891,859, U.S. Pat. No.6,417,428, U.S. Pat. No. 6,664,446, U.S. Pat. No. 6,706,866, U.S. Pat.No. 6,717,034, U.S. Pat. No. 6,801,104, WO2000060089, WO2001026459,WO2001035725, WO2001034726, WO2001035727, WO2001036444, WO2001036597,WO2001036598, WO2002015675, WO2002017430, WO2002077185, WO2002079403,WO2003013227, WO2003013228, WO2003014327, WO2004031349, WO2004076638,WO9809521, and WO9938977 describing genes, including CBF genes andtranscription factors effective in mitigating the negative effects offreezing, high salinity, and drought on plants, as well as conferringother positive effects on plant phenotype; US2004/0148654 and WO01/36596where abscisic acid is altered in plants resulting in improved plantphenotype such as increased yield and/or increased tolerance to abioticstress; WO2000/006341, WO04/090143, U.S. application Ser. Nos.10/817,483 and 09/545,334 where cytokinin expression is modifiedresulting in plants with increased stress tolerance, such as droughttolerance, and/or increased yield. Also see WO0202776, WO2003052063,JP2002281975, U.S. Pat. No. 6,084,153, WO0164898, U.S. Pat. No.6,177,275, and U.S. Pat. No. 6,107,547 (enhancement of nitrogenutilization and altered nitrogen responsiveness). For ethylenealteration, see US20040128719, US20030166197 and W0200032761. For planttranscription factors or transcriptional regulators of abiotic stress,see e.g. US20040098764 or US20040078852.

Other genes and transcription factors that affect plant growth andagronomic traits such as yield, flowering, plant growth and/or plantstructure, can be introduced or introgressed into plants, see e.g.WO97/49811 (LHY), WO98/56918 (ESD4), WO97/10339 and U.S. Pat. No.6,573,430 (TFL), U.S. Pat. No. 6,713,663 (FT), WO96/14414 (CON),WO96/38560, WO01/21822 (VRN1), WO00/44918 (VRN2), WO99/49064 (GI),WO00/46358 (FRI), WO97/29123, U.S. Pat. No. 6,794,560, U.S. Pat. No.6,307,126 (GAI), WO99/09174 (D8 and Rht), and WO2004076638 andWO2004031349 (transcription factors).

7. Genes that Confer Agronomic Enhancements, Nutritional Enhancements,or Industrial Enhancements.

(A) Improved tolerance to water stress from drought or high salt watercondition. The HVA1 protein belongs to the group 3 LEA proteins thatinclude other members such as wheat pMA2005 (Curry et al., 1991; Curryand Walker-Simmons, 1993), cotton D-7 (Baker et al., 1988), carrot Dc3(Seffens et al., 1990), and rape pLEA76 (Harada et al., 1989). Theseproteins are characterized by 11-mer tandem repeats of amino aciddomains which may form a probable amphophilic alpha-helical structurethat presents a hydrophilic surface with a hydrophobic stripe (Baker etal., 1988; Dure et al., 1988; Dure, 1993). The barley HVA1 gene and thewheat pMA2005 gene (Curry et al., 1991; Curry and Walker-Simmons, 1993)are highly similar at both the nucleotide level and predicted amino acidlevel. These two monocot genes are closely related to the cotton D-7gene (Baker et al., 1988) and carrot Dc3 gene (Seffens et al., 1990)with which they share a similar structural gene organization (Straub etal., 1994). There is, therefore, a correlation between LEA geneexpression or LEA protein accumulation with stress tolerance in a numberof plants. For example, in severely dehydrated wheat seedlings, theaccumulation of high levels of group 3 LEA proteins was correlated withtissue dehydration tolerance (Ried and Walker-Simmons, 1993). Studies onseveral Indica varieties of rice showed that the levels of group 2 LEAproteins (also known as dehydrins) and group 3 LEA proteins in rootswere significantly higher in salt-tolerant varieties compared withsensitive varieties (Moons et al., 1995). The barley HVA1 gene wastransformed into wheat. Transformed wheat plants showed increasedtolerance to water stress, (Sivamani, E. et al. Plant Science 2000, V.155 p 1-9 and U.S. Pat. No. 5,981,842.)

(B) Another example of improved water stress tolerance is throughincreased mannitol levels via the bacterial mannitol-1-phosphatedehydrogenase gene. To produce a plant with a genetic basis for copingwith water deficit, Tarczynski et al. (Proc. Natl. Acad. Sci. USA, 89,2600 (1992); WO 92/19731, published No. 12, 1992; Science, 259, 508(1993)) introduced the bacterial mannitol-1-phosphate dehydrogenasegene, mtlD, into tobacco cells via Agrobacterium-mediatedtransformation. Root and leaf tissues from transgenic plants regeneratedfrom these transformed tobacco cells contained up to 100 mM mannitol.Control plants contained no detectable mannitol. To determine whetherthe transgenic tobacco plants exhibited increased tolerance to waterdeficit, Tarczynski et al. compared the growth of transgenic plants tothat of untransformed control plants in the presence of 250 mM NaCl.After 30 days of exposure to 250 mM NaCl, transgenic plants haddecreased weight loss and increased height relative to theiruntransformed counterparts. The authors concluded that the presence ofmannitol in these transformed tobacco plants contributed to waterdeficit tolerance at the cellular level. See also U.S. Pat. No.5,780,709 and international publication WO 92/19731 which areincorporated herein by reference for this purpose.

Numerous methods for plant transformation have been developed, includingbiological and physical, plant transformation protocols. See, forexample, Miki et al., “Procedures for Introducing Foreign DNA intoPlants” in Methods in Plant Molecular Biology and Biotechnology, Glick,B. R. and Thompson, J. E. Eds. (CRC Press, Inc., Boca Raton, 1993) pages67-88. In addition, expression vectors and in vitro culture methods forplant cell or tissue transformation and regeneration of plants areavailable. See, for example, Gruber et al., “Vectors for PlantTransformation” in Methods in Plant Molecular Biology and Biotechnology,Glick, B. R. and Thompson, J. E. Eds. (CRC Press, Inc., Boca Raton,1993) pages 89-119.

Further embodiments of the invention are the treatment of W030258E2 witha mutagen and the plant produced by mutagenesis of W030258E2.Information about mutagens and mutagenizing seeds or pollen arepresented in the IAEA's Manual on Mutation Breeding (IAEA, 1977) otherinformation about mutation breeding in wheat can be found in C. F.Konzak, “Mutations and Mutation Breeding” chapter 7B, of Wheat and WheatImprovement, 2^(nd) edition, ed. Heyne, 1987.

A further embodiment of the invention is a backcross conversion of wheatvariety W030258E2. A backcross conversion occurs when DNA sequences areintroduced through traditional (non-transformation) breeding techniques,such as backcrossing. DNA sequences, whether naturally occurring ortransgenes, may be introduced using these traditional breedingtechniques. Desired traits transferred through this process include, butare not limited to nutritional enhancements, industrial enhancements,disease resistance, insect resistance, herbicide resistance, agronomicenhancements, grain quality enhancement, waxy starch, breedingenhancements, seed production enhancements, and male sterility.Descriptions of some of the cytoplasmic male sterility genes, nuclearmale sterility genes, chemical hybridizing agents, male fertilityrestoration genes, and methods of using the aforementioned are discussedin “Hybrid Wheat by K. A. Lucken (pp. 444-452 In Wheat and WheatImprovement, ed. Heyne, 1987). Examples of genes for other traitsinclude: Leaf rust resistance genes (Lr series such as Lr1, Lr10, Lr21,Lr22, Lr22a, Lr32, Lr37, Lr41, Lr42, and Lr43), Fusarium headblight-resistance genes (QFhs.ndsu-3B and QFhs.ndsu-2A), Powdery Mildewresistance genes (Pm21), common bunt resistance genes (Bt-10), and wheatstreak mosaic virus resistance gene (Wsm1), Russian wheat aphidresistance genes (Dn series such as Dn1, Dn2, Dn4, Dn5), Black stem rustresistance genes (Sr38), Yellow rust resistance genes (Yr series such asYr1, YrSD, Yrsu, Yr17, Yr15, YrH52), Aluminum tolerance genes (Alt(BH)),dwarf genes (Rht), vernalization genes (Vrn), Hessian fly resistancegenes (H9, H10, H21, H29), grain color genes (R/r), glyphosateresistance genes (EPSPS), glufosinate genes (bar, pat) and water stresstolerance genes (Hva1, mtlD). The trait of interest is transferred fromthe donor parent to the recurrent parent, in this case, the wheat plantdisclosed herein. Single gene traits may result from either the transferof a dominant allele or a recessive allele. Selection of progenycontaining the trait of interest is done by direct selection for a traitassociated with a dominant allele. Selection of progeny for a trait thatis transferred via a recessive allele requires growing and selfing thefirst backcross to determine which plants carry the recessive alleles.Recessive traits may require additional progeny testing in successivebackcross generations to determine the presence of the gene of interest.

Another embodiment of this invention is a method of developing abackcross conversion W030258E2 wheat plant that involves the repeatedbackcrossing to wheat variety W030258E2. The number of backcrosses mademay be 2, 3, 4, 5, 6 or greater, and the specific number of backcrossesused will depend upon the genetics of the donor parent and whethermolecular markers are utilized in the backcrossing program. See, forexample, R. E. Allan, “Wheat” in Principles of Cultivar Development,Fehr, W. R. Ed. (Macmillan Publishing Company, New York, 1987) pages722-723, incorporated herein by reference. Using backcrossing methods,one of ordinary skill in the art can develop individual plants andpopulations of plants that retain at least 70%, 75%, 79%, 80%, 81%, 82%,83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%,97%, 98%, or 99% of the genetic profile of wheat variety W030258E2. Thepercentage of the genetics retained in the backcross conversion may bemeasured by either pedigree analysis or through the use of genetictechniques such as molecular markers or electrophoresis. In pedigreeanalysis, on average 50% of the starting germplasm would be passed tothe progeny line after one cross to another line, 75% after backcrossingonce, 87.5% after backcrossing twice, and so on. These percentages areaverages and each individual progeny plant may have a differentpercentage of the parental genome after each cross and/or backcross. Andusing molecular markers one could determine individuals and selectindividuals that have a much higher percentage of the recurrent parentat each stage of the backcross process. Molecular markers could also beused to confirm and/or determine the recurrent parent used. Molecularmarker assisted breeding or selection may be utilized to reduce thenumber of backcrosses necessary to achieve the backcross conversion. Forexample, see Openshaw, S. J. et al., Marker-assisted Selection inBackcross Breeding, In: Proceedings Symposium of the Analysis ofMolecular Data, August 1994, Crop Science Society of America, Corvallis,Oreg., where it is demonstrated that a locus conversion can be made inas few as two backcrosses. The backcross conversion or locus conversiondeveloped from this method may be similar to W030258E2 for the resultslisted in Table 1. Such similarity may be measured by a side by sidephenotypic comparison, with differences and similarities determined at a5% significance level. Any such comparison should be made inenvironmental conditions that account for the trait being transferred.For example, herbicide should not be applied in the phenotypiccomparison of herbicide resistant backcross conversion of W030258E2 whencompared back to W030258E2.

Another embodiment of the invention is an essentially derived variety ofW030258E2 or a locus conversion of W030258E2. As determined by the UPOVConvention, essentially derived varieties may be obtained for example bythe selection of a natural or induced mutant, or of a somaclonalvariant, the selection of a variant individual from plants of theinitial variety, backcrossing, or transformation by genetic engineering.An essentially derived variety of W030258E2 is further defined as onewhose production requires the repeated use of variety W030258E2 or ispredominately derived from variety W030258E2. International Conventionfor the Protection of New Varieties of Plants, as amended on Mar. 19,1991, Chapter V, Article 14, Section 5(c). A locus conversion refers toplants within a variety that have been modified in a manner that retainsthe overall genetics of the variety and further comprises one or moreloci with a specific desired trait, such as male sterility, insect,disease or herbicide resistance. Examples of single locus conversionsinclude mutant genes, transgenes and native traits finely mapped to asingle locus. One or more locus conversion traits may be introduced intoa single wheat variety. As used herein, the phrase ‘comprising a’transgene, transgenic event or locus conversion means one or moretransgenes, transgenic events or locus conversions.

This invention also is directed to methods for using wheat varietyW030258E2 in plant breeding.

One such embodiment is the method of crossing wheat variety W030258E2with another variety of wheat to form a first generation population ofF1 plants. The population of first generation F1 plants produced by thismethod is also an embodiment of the invention. This first generationpopulation of F1 plants will comprise an essentially complete set of thealleles of wheat variety W030258E2. One of ordinary skill in the art canutilize either breeder books or molecular methods to identify aparticular F1 plant produced using wheat variety W030258E2, and any suchindividual plant is also encompassed by this invention. Theseembodiments also cover use of transgenic or backcross conversions ofwheat variety W030258E2 to produce first generation F1 plants.

A method of developing a W030258E2-progeny wheat plant comprisingcrossing W030258E2 with a second wheat plant and performing a breedingmethod is also an embodiment of the invention. A specific method forproducing a line derived from wheat variety W030258E2 is as follows. Oneof ordinary skill in the art would cross wheat variety W030258E2 withanother variety of wheat, such as an elite variety. The F1 seed derivedfrom this cross would be grown to form a homogeneous population. The F1seed would contain one set of the alleles from variety W030258E2 and oneset of the alleles from the other wheat variety. The F1 genome would bemade-up of 50% variety W030258E2 and 50% of the other elite variety. TheF1 seed would be grown and allowed to self, thereby forming F2 seed. Onaverage the F2 seed would have derived 50% of its alleles from varietyW030258E2 and 50% from the other wheat variety, but various individualplants from the population would have a much greater percentage of theiralleles derived from W030258E2 (Wang J. and R. Bernardo, 2000, Crop Sci.40:659-665 and Bernardo, R. and A. L. Kahler, 2001, Theor. Appl. Genet102:986-992). The F2 seed would be grown and selection of plants wouldbe made based on visual observation and/or measurement of traits. TheW030258E2-derived progeny that exhibit one or more of the desiredW030258E2-derived traits would be selected and each plant would beharvested separately. This F3 seed from each plant would be grown inindividual rows and allowed to self. Then selected rows or plants fromthe rows would be harvested and threshed individually. The selectionswould again be based on visual observation and/or measurements fordesirable traits of the plants, such as one or more of the desirableW030258E2-derived traits. The process of growing and selection would berepeated any number of times until a homozygous W030258E2-derived wheatplant is obtained. The homozygous W030258E2-derived wheat plant wouldcontain desirable traits derived from wheat variety W030258E2, some ofwhich may not have been expressed by the other original wheat variety towhich wheat variety W030258E2 was crossed and some of which may havebeen expressed by both wheat varieties but now would be at a level equalto or greater than the level expressed in wheat variety W030258E2. Thehomozygous W030258E2-derived wheat plants would have, on average, 50% oftheir genes derived from wheat variety W030258E2, but various individualplants from the population would have a much greater percentage of theiralleles derived from W030258E2. The breeding process, of crossing,selfing, and selection may be repeated to produce another population ofW030258E2-derived wheat plants with, on average, 25% of their genesderived from wheat variety W030258E2, but various individual plants fromthe population would have a much greater percentage of their allelesderived from W030258E2. Another embodiment of the invention is ahomozygous W030258E2-derived wheat plant that has receivedW030258E2-derived traits.

The modified pedigree selection method of breeding was used to derivethis line from elite germplasm. The first cross was made in 1996 andbreeding continued until August 2008. W030258E2 represents a significantadvancement in elite germplasm adapted to the United States.

The previous example can be modified in numerous ways, for instanceselection may or may not occur at every selfing generation, selectionmay occur before or after the actual self-pollination process occurs, orindividual selections may be made by harvesting individual spikes,plants, rows or plots at any point during the breeding processdescribed. In addition, double haploid breeding methods may be used atany step in the process. The population of plants produced at each andany generation of selfing is also an embodiment of the invention, andeach such population would consist of plants containing approximately50% of its genes from wheat variety W030258E2, 25% of its genes fromwheat variety W030258E2 in the second cycle of crossing, selfing, andselection, 12.5% of its genes from wheat variety W030258E2 in the thirdcycle of crossing, selfing, and selection, and so on.

Another embodiment of this invention is the method of obtaining ahomozygous W030258E2-derived wheat plant by crossing wheat varietyW030258E2 with another variety of wheat and applying double haploidmethods to the F1 seed or F1 plant or to any generation ofW030258E2-derived wheat obtained by the selfing of this cross.

Still further, this invention also is directed to methods for producingW030258E2-derived wheat plants by crossing wheat variety W030258E2 witha wheat plant and growing the progeny seed, and repeating the crossingor selfing along with the growing steps with the W030258E2-derived wheatplant from 1 to 2 times, 1 to 3 times, 1 to 4 times, or 1 to 5 times.Thus, any and all methods using wheat variety W030258E2 in breeding arepart of this invention, including selfing, pedigree breeding,backcrossing, hybrid production and crosses to populations. Uniquestarch profiles, molecular marker profiles and/or breeding records canbe used by those of ordinary skill in the art to identify the progenylines or populations derived from these breeding methods.

Another embodiment of this invention is the method of harvesting thegrain of variety wheat variety W030258E2 and using the grain as seed forplanting. Embodiments include cleaning the seed, treating the seed,and/or conditioning the seed. Cleaning the seed includes removingforeign debris such as weed seed and removing chaff, plant matter, fromthe seed. Conditioning the seed can include controlling the temperatureand rate of dry down and storing seed in a controlled temperatureenvironment. Seed treatment is the application of a composition to theseed such as a coating or powder. Seed material can be treated,typically surface treated, with a composition comprising combinations ofchemical or biological herbicides, herbicide safeners, pesticides,insecticides, fungicides, nutrients, germination inhibitors, germinationpromoters, cytokinins, nutrients, plant growth regulators,antimicrobials, and activators, bactericides, nematicides, avicides, ormolluscicides. These compounds are typically formulated together withfurther carriers, surfactants or application-promoting adjuvantscustomarily employed in the art of formulation. The coatings may beapplied by impregnating propagation material with a liquid formulationor by coating with a combined wet or dry formulation. Examples of thevarious types of compounds that may be used as seed treatments areprovided in The Pesticide Manual: A World Compendium, C. D. S. TomlinEd., Published by the British Crop Production Council. Some specificseed treatments that may be used on crop seed include, but are notlimited to, abscisic acid, acibenzolar-S-methyl, avermectin, amitrol,azaconazole, azospirillum, azoxystrobin, bacillus, Bacillus subtilis,Bacillus simplex, Bacillus firmus, Bacillus amyloliquefaciens, Pasteuriagenus (e.g. P. nishizawae), bradyrhizobium, captan, carboxin, chitosan,clothianidin, copper, cyazypyr, difenoconazole, etidiazole, fipronil,fludioxonil, fluquinconazole, flurazole, fluxofenim, GB126, Harpinprotein, imazalil, imidacloprid, ipconazole, isofavenoids,lipo-chitooligosaccharide, mancozeb, manganese, maneb, mefenoxam,metalaxyl, metconazole, PCNB, penflufen, penicillium, penthiopyrad,permethrine, picoxystrobin, prothioconazole, pyraclostrobin, rynaxypyr,S-metolachlor, saponin, sedaxane, TCMTB, tebuconazole, thiabendaxole,thiamethoxam, thiocarb, thiram, tolclofos-methyl, triadimenol,trichoderma, trifloxystrobin, triticonazole and/or zinc.

It is routine practice to test seed varieties and seeds with specificgenetic resistance traits to determine which seed treatment options andapplication rates will complement such varieties and genetic resistancetraits in order to enhance yield. For example, a variety with good yieldpotential but loose smut susceptibility will benefit from the use of aseed treatment that provides protection against loose smut. Likewise, avariety encompassing a genetic resistance trait conferring insectresistance will benefit from the second mode of action conferred by theseed treatment. Further, the good root establishment and early emergencethat results from the proper use of a seed treatment will result in moreefficient nitrogen use, a better ability to withstand drought and anoverall increase in yield potential of a variety or varieties containinga certain trait when combined with a seed treatment.Performance Examples of W030258E2

In the examples that follow, the traits and characteristics of wheatvariety W030258E2 are given in paired comparisons with another varietyduring the same growing conditions and same year. The data collected oneach wheat variety is presented for a number of characteristics andtraits. (Table 2, Table 3, and Table 4).

The results in Table 2 compare variety W030258E2 to varieties 25R47,25R42, and 25R40 for various agronomic traits. Data in Table 2 wascollected at locations in Arkansas, Georgia, Illinois, Indiana,Kentucky, Michigan, Missouri, Mississippi, North Carolina, Ohio,Tennessee, Virginia, and Ontario, Canada. The results in Table 3 comparevariety W030258E2 to varieties 25R47, 25R32, and 25R40 for variousdisease resistance traits. Data in Table 3 was collected at locations inArkansas, Georgia, Illinois, Indiana, Kentucky, Michigan, Missouri,Mississippi, North Carolina, Ohio, Tennessee, Virginia, and Ontario,Canada. The results in Table 4 show values for the grain quality ofvariety W030258E2 and comparison varieties 25R47, 25R32 and 25R40.Quality data were collected from 2010-2012 at the USDA-ARS Soft WheatQuality Lab in Wooster, Ohio.

TABLE 2 Agronomic trait paired comparisons of W030258E2 during theperiod 2009-2012. Heading Grain Test Plant Date Straw Yield WeightHeight After Lodging Variety bu/ac lb/bu cm Jan 1 1-9@ 2009-12 W030258E292.4 58.9 81.3 124.0 7.0 25R47 92.0 57.2 83.8 123.0 7.0 Locations 49 509 17 4 Reps. 95 97 16 33 8 Prob. 0.8154 0.0000 0.1782 0.0203 0.71772011-12 W030258E2 88.0 59.1 83.8 112.0 7.0 25R32 87.1 60.2 88.9 114.05.0 Locations 35 36 5 9 4 Reps. 68 70 8 16 8 Prob. 0.6645 0.0004 0.04850.0939 0.0436 2010-12 W030258E2 89.8 58.9 81.3 120.0 7.0 25R40 93.5 59.281.3 121.0 8.0 Locations 43 44 7 15 4 Reps. 88 89 13 30 8 Prob. 0.04140.0852 0.8868 0.0628 0.3751 @Scale of 1-9 where 9 = excellent orresistant, 1 = poor or susceptible.Data in above table collected at locations in Arkansas, Georgia,Illinois, Indiana, Kentucky, Michigan, Missouri, Mississippi, NorthCarolina, Ohio, Tennessee, Virginia, and Ontario, Canada.

TABLE 3 Disease trait paired comparisons of W030258E2 during the period2009-2012. Leaf Leaf Stripe Powdery Rust Blight Scab Rust Mildew SBMVVariety 1-9 @ 1-9 @ 1-9 @ 1-9 @ 1-9 @ 1-9 @ 2009-12 W030258E2 7.0 7.06.0 5.0 4.0 4.0 25R47 6.0 5.0 4.0 8.0 4.0 6.0 Locations 11 1 7 8 5 2Reps. 21 2 13 14 8 3 Prob. 0.0382 0.0006 0.0003 0.2835 0.2048 2011-12W030258E2 7.0 7.0 5.0 4.0 4.0 25R32 5.0 7.0 8.0 7.0 6.0 Locations 10 4 83 1 Reps. 20 8 14 5 2 Prob. 0.0014 0.0917 0.0008 0.2697 2010-12W030258E2 7.0 7.0 7.0 5.0 4.0 4.0 25R40 6.0 6.0 5.0 8.0 8.0 5.0Locations 11 1 6 8 3 2 Reps. 21 2 12 14 5 3 Prob. 0.0883 0.0021 0.00180.1843 0.2048 @ Scale of 1-9 where 9 = excellent or resistant, 1 = pooror susceptible. SSMV = Wheat Spindle Streak Mosaic Virus SBMV =Soil-borne Mosaic Virus.Data in above table collected at locations in Arkansas, Georgia,Illinois, Indiana, Kentucky, Michigan, Missouri, Mississippi, NorthCarolina, Ohio, Tennessee, Virginia, and Ontario, Canada.

TABLE 4 Average Soft wheat quality data, 2010-2012. Break Lactic FlourFlour Flour Acid Sucrose Yield Yield Protein SRC SRC Variety % % % % %2010-12 W030258E2 73.7 35.1 6.8 81.0 81.1 25R47 71.6 46.7 6.8 87.2 82.5Years 3 3 3 3 3 Reps. 3 3 3 3 3 Prob. 0.1648 0.0397 0.9942 0.2416 0.42392011-12 W030258E2 73.1 34.1 7.8 73.7 82.5 25R32 73.2 36.2 8.0 83.3 81.0Years 2 2 2 2 2 Reps. 2 2 2 2 2 Prob. 0.8385 0.3036 0.1352 0.2114 0.57682011-12 W030258E2 73.1 34.1 7.8 73.7 82.5 25R40 69.6 44.4 7.3 91.7 88.4Years 2 2 2 2 2 Reps. 2 2 2 2 2 Prob. 0.0775 0.1038 0.3292 0.1423 0.0973Lactic Acid SRC = Lactic Acid Solvent Retention Capacity Sucrose SRC =Sucrose solution Retention Capacity Quality data collected at theUSDA-ARS Soft Wheat Quality Lab in Wooster, OHExamples of Assays Performed to Develop W030258E2

The following examples provide descriptions of several assays that canbe used to characterize and/or select a wheat variety during one or morestages of variety development. Many other methods and assays areavailable and can be substituted for, or used in combination with, oneor more of the examples provided herein. Tables 1, 2, 3 and 4 providefurther information on wheat variety W030258E2, which results may beproduced from at least one or more assays or methods described in thefollowing examples.

Example 1 Stripe Rust Screening

Stripe rust is a fungal leaf disease that is most common in themid-southern United States in the early spring. Significant levels ofthe disease can be found in some seasons anywhere in North America. Theinfection often mostly occurs on the flag leaf but it may attack theentire plant, including the head. Natural infection of plants in thefield may be rated visually using a 1-9 scale, where 1 indicatescomplete susceptibility and 9 indicates complete resistance. Some majorgenes for resistance may be detected using controlled seedling screeningexperiments inoculated with specific races of the pathogen. There arealso molecular markers for QTL linked to some specific resistance genes.

Example 2 Leaf Rust Screening

Leaf rust is a fungal leaf disease that is most common in the southernUnited States in the spring and early summer. Significant levels of thedisease can be found in most seasons anywhere in North America. Theinfection is most damaging when it occurs on the flag leaf but it mayattack the entire plant, including the head. Natural infection of plantsin the field may be rated visually using a 1-9 scale, where 1 indicatescomplete susceptibility and 9 indicates complete resistance. Some majorgenes for resistance may be detected using controlled seedling screeningexperiments inoculated with specific races of the pathogen. There arealso molecular markers for QTL linked to some specific resistance genes.

Example 3 Leaf Blight Screening

Fungal leaf blights, including Tan spot, Septoria tritici blotch, andStagnospora nodorum blotch, are common in much of the North Americanwheat growing regions. The infection is most damaging when it occurs onthe flag leaf but it may attack the entire plant, including the head.Natural infection of plants in the field may be rated visually using a1-9 scale, where 1 indicates complete susceptibility and 9 indicatescomplete resistance.

Example 4 Scab Screening

Fusarium head blight or scab is a fungal disease that is common in muchof the North American wheat growing regions. Infection occurs duringflowering and is most severe when conditions are wet, warm and remainhumid. The disease infects flowers on the spike and will spread toadjacent flowers, often infecting most of the developing kernels on thespike. Natural infection of plants in the field may be rated visuallyusing a 1-9 scale, where 1 indicates complete susceptibility and 9indicates complete resistance. Infection may be induced in controlledscreening experiments where spikes are inoculated with specific sporeconcentrations of the fungus by spraying the spikes at flowering orinjecting the inoculum directly into a flower on each spike. There arealso molecular markers for QTL linked to some specific resistance genes.

Example 5 Powdery Mildew Screening

Powdery mildew is a fungal leaf disease that is most common in thesouthern United States in the spring and early summer. Significantlevels of the disease can be found in many seasons anywhere in NorthAmerica. The infection is most damaging when it occurs on the flag leafbut it may attack the entire plant, including the head. Naturalinfection of plants in the field may be rated visually using a 1-9scale, where 1 indicates complete susceptibility and 9 indicatescomplete resistance. Some major genes for resistance may be detectedusing controlled seedling screening experiments inoculated with specificraces of the pathogen. There are also molecular markers for QTL linkedto some specific resistance genes.

Example 6 Soilborne Mosaic Virus Screening

Soilborne mosaic virus is transmitted by the vector, Polymyxa graminis,which tends to be most common in low-lying, wet soils; particularlythose frequently grown to wheat. Symptoms appear in the spring as lightgreen to yellow mottling along with stunting and resetting plant growthin the most susceptible varieties. Natural infection of plants in thefield may be rated visually using a 1-9 scale, where 1 indicatescomplete susceptibility and 9 indicates complete resistance. Higherlevels of natural infection can be induced for screening by plantingwheat annually in the same field to increase the vector level.

Example 7 Wheat Yellow (Spindle Streak) Mosaic Virus Screening

Wheat yellow virus is transmitted by the vector, Polymyxa graminis, andis most common during cool weather conditions in the spring. Symptomsappear as light green to yellow streaks and dashes parallel to the leafveins. Symptoms often fade prior to heading as weather conditions becomewarmer. Natural infection of plants in the field may be rated visuallyusing a 1-9 scale, where 1 indicates complete susceptibility and 9indicates complete resistance.

Example 8 Flour Yield Screening

The potential average flour yield of wheat can be determined on samplesof grain that has been cleaned to standard and tempered to uniformmoisture, using a test mill such as the Allis-Chalmers or Brabendermill. Samples are milled to established parameters, the flour siftedinto fractions, which are then weighed to calculate flour yield as apercentage of grain weight.

Flour yield “as is” is calculated as the bran weight (over 40 weight)subtracted from the grain weight, divided by grain weight and times 100to equal “as is” flour yield. Flour yield is calculated to a 15% grainmoisture basis as follows: flour moisture is regressed to predict thegrain moisture of the wheat when it went into the Quad Mill using theformulaInitial grain moisture=1.3429×(flour moisture)−4.The flour yields are corrected back to 15% grain moisture afterestimating the initial grain moisture using the formulaFlour Yield_((15%))=Flour Yield_((as is))−1.61%×(15%−Actual flourmoisture)

Example 9 Flour Protein Screening

The protein content as a percentage of total flour may be estimated bythe Kjeldahl method or properly calibrated near-infrared reflectanceinstruments to determine the total nitrogen content of the flour.

Flour protein differences among cultivars can be a reliable indicator ofgenetic variation provided the varieties are grown together, but canvary from year to year at any given location. Flour protein from asingle, non-composite sample may not be representative. Based on theSoft Wheat Quality Laboratory grow-outs, protein can vary as much 1.5%for a cultivar grown at various locations in the same ½ acre field.

Example 10 Sucrose Solvent Retention Capacity (SRC)

The solvent retention capacity (SRC) of wheat flour measures the abilityof the flour to retain various solvents after centrifugation. SucroseSRC predicts the starch damage and pentosan components, and can becorrelated to sugar-snap cookie diameter quality metrics.

Sucrose SRC is a measure of arabinoxylans (also known as pentosans)content, which can strongly affect water absorption in baked products.Water soluble arabinoxylans are thought to be the fraction that mostgreatly increases sucrose SRC. Sucrose SRC probably is the bestpredictor of cookie quality, with sugar snap cookie diameters decreasingby 0.07 cm for each percentage point increase in sucrose SRC. Thenegative correlation between wire-cut cookie and sucrose SRC values isr=−0.66 (p<0.0001). Sucrose SRC typically increases in wheat sampleswith lower flour yield (r=−0.31) and lower softness equivalent(r=−0.23). The cross hydration of gliadins by sucrose also causessucrose SRC values to be correlated to flour protein (r=0.52) and lacticacid SRC (r=0.62). Soft wheat flours for cookies typically have a targetof 95% or less when used by the US baking industry for biscuits andcrackers. Sucrose SRC values increase by 1% for every 5% increase inlactic acid SRC. The 95% target value can be exceeded in flour sampleswhere a higher lactic acid SRC is required for product manufacture sincethe higher sucrose SRC is due to gluten hydration and not to swelling ofthe water soluble arabinoxylans.

Example 11 Lactic Acid SRC

Lactic Acid SRC=Lactic Acid Solvent Retention Capacity. Lactic acid SRCmeasures gluten strength. Typical values are below 85% for “weak” softvarieties and above 105% or 110% for “strong” gluten soft varieties. Seethe above discussion of protein quality in this section for additionaldetails of the lactic acid SRC. Lactic acid SRC results correlate to theSDS-sedimentation test. The lactic acid SRC is also correlated to flourprotein concentration, but the effect is dependent on genotypes andgrowing conditions. The SWQL typically reports a protein-correctedlactic acid SRC value to remove some of the inherent protein fluctuationnot due to cultivar genetics. Lactic acid is corrected to 9% proteinusing the assumption of a 7% increase in lactic acid SRC for every 1%increase in flour protein. On average across 2007 and 2008, the changein lactic acid SRC value was closer to 2% for every 1% protein.

Example 12 Molecular Screening

As shown in Table 1, plants were analyzed at various times throughoutthe development of W030258E2 for specific alleles for scab resistance.As discussed above, and as is known to those skilled in the art, othertraits can also be screened by molecular analysis.

Table 5 lists common traits and a description of how the trait isscored.

TABLE 5 TRAIT DESCRIPTION & HOW SCORED HD DAT Heading Date in days pastJan. 1st); plot dated on the day when approximately 50% of the heads are50% out of the boot HGTIN Height (inches or centimeters); scored with ameasuring stick HGTCM after all genotypes fully extended; wheat gatheredaround stick and average distance to the top of the heads is noted; 2-3samplings per plot LF BLT Leaf Blight Complex; score based on amount ofinfection on flag and flag −1 leaves; typical scale: % of uninfectedleaf surface area flag flag −1 9 - 100% 100% 8 - 100%  75% 7 - 100%  50%6 - >90% <50% 5 - 75-90% <25% 4 - 50-74% — 3 - 23-49% — 2 - 10-24% — 1 -0-9% — LF RST Leaf Rust; score based on amount of infection evident onflag leaves; typical scale: 9 - clean 8 - trace amounts 7 - <5% flagleaf area infected 6 - 6-10% ″ 5 - 11-20% ″ 4 - 21-30% ″ 3 - 31-40% ″2 - 41-50% ″ 1 - over 50% ″ MAT Maturity; used on larger, earliergeneration tests in the place of heading date; scale based on maturityof known checks and will vary from year to year based on when the noteis taken; typical scale: 9 - very late, boot not swelling when note istaken 8 - still in boot when note is taken 7 - splitting boot, will headtwo days after note is taken 6 - will head day after the note is taken5 - headed on the day note is taken 4 - headed day before note taken 3 -headed two days before note taken 2 - fully extended, some floweringvisible 1 - extended and flowering Maturity may also be scored atphysiological maturity; typical scaler: 9 - ready to be harvested 7 -caryopse hard to divide 5 - head yellowing an day note is taken 3 -grain still at dough stage 1 - head completely green PM Powdery Mildew;score based on severity of infection and progression of the disease upthe plant; scale based on reaction of known checks with attention givento race changes; typical scale: 9 - clean 8 - trace amount low on plants7 - slight infection mostly low on plants 6 - moderate infection low onplants; trace amounts on flag −1 leaves 5 - moderate infection low onplants, moderate amounts on flag −1 leaves 4 - moderate infectionthrough canopy with trace amounts evident on flag leaves 3 - severeinfection through canopy with up to 25% infection on flag leaves 2 -severe infection through canopy with up to 50% infection on flag leaves1 - severe infection; greater than 50% infection on flag leaves SB MVSoil Borne Mosaic Virus; score based on amount of mottling, ohlorosis,and/or stunting; scale based on reaction of known checks; typical scale1 - severe stunting to the point of rosettes 2 - severe stunting 3 -very chlorotic with moderate stunting 4 - very chlorotic with mildstunting 5 - moderate mottling with no stunting 6 - mottling evident 7 -mottling barely visible 8 - green, very little mottling 9 - green, nomottling visible SHTSC Shattering score. Scores are based on the amountof grain that is visible in the spike just before harvest. 9 - grain novisible in the spike, Glumes closed. 8 - Glumes slightly opened in <10%of the grains. 7 - Glumes slightly opened in >10% of the grains. 6 -Glumes moderately opened in <20% of the grains. 5 - Glumes moderatelyopened in >20% of the grains. 4 - Glumes completely opened in <30% ofthe grains. 3 - Glumes completely opened in >30% of the grains. 2 -20%-50% of the grain on the soil 1 - >50% of the grain on the soil. SSMV Spindle Streak Mosaic Virus; score based on amount of mottling andchlorosis; scale based on reaction of known checks; scale similar to SSMV with less emphasis on stunting ST EDG Straw Lodging; score based onamount of lodging; typical scale: 9 - still upright 8 - only slightleaning 7 - some leaning, no lodging 6 - moderate leaning, littlelodging 5 - up to 10% lodged 4 - 11-25% lodged 3 - 26-50% lodged 2 -51-75% lodged 1 - greater than 75% lodged STPRST Stripe rust. Striperust is an important disease that occurs most often in Europe. Theinfection may only affect the flag leaf, or it may attack the entireplant including the head. Two scales based on level of infectionincluded below: Score based on the amount of infection of the wholeplant! 9 - clean 8 - traces 7 - <5% plant infected 6 - 10% plantinfected 5 - 20% plant infected 4 - 40% plant infected 3 - 60% plantinfected 2 - 60% plant infected head rusted 1 - Plant not able toproduce kernel Score based on the amount and type of infection evidenton flag leaves: 9 - clean 8 - trace amounts (Chlorotic-necroticfreckles) 7 - <5% flag leaf area infected 6 - 6-10% ″(chlorotic-necrotic stripes). 5 - 11-20% ″ (chlorotic-necrotic stripes).4 - 21-30% ″ (chlorotic-necrotic stripes). 3 - 31-40% ″(chlorotic-necrotic stripes). 2 - 41-50% ″ (some chlorosis). 1 - over50% ″ (no chlorosis). UNI Uniformity; used to determine how pure a lineis generally at the F7 (pre-advanced) generation; typical scale: 9 -very uniform in all aspects 8 - good uniformity 7 - fairly uniform, butsome off-types 6 - several off-types, but can be cleaned up with normalpurification procedures 5 - several off-types, will be a challenge toclean up with normal purification procedures 4 - considerable number ofoff-types; will need to be reselected to proceed as a pureline 3 - asmany as 25% off types; will need to be reselected 2 - as many as 50% offtypes; will need to be reselected 1 - more than 50% off types; what youhave here is a problem WNTHRD Winter Hardiness; score based on amount ofbrownback and kill; best scored at time of early spring regrowth;typical scale: 9 - very green, no brown-back 8 - green, slightbrown-back 7 - moderate brown-back 6 - hard brown-back, no kill 5 - hardbrown-back with less than 10% kill 4 - 11-25% kill 3 - 26-50% kill 2 -51-75% kill 1 - greater than 75% kill SC AB fusarium head scab; scorebased on visual evaluation of the percentage of scab infected heads on awhole plot basis with consideration given to both total heads affectedand severity of infection; typical scale: 9 - no scab infection 8 -trace amount (1-2%) with infections limited to individual spikelets 7 -up to 5% infection with most infection limited to less than 50% of thespike 6 - 5-15% of heads infected 5 - 15-30% of heads infected 4 -30-50% of heads infected 3 - 50-75% of heads infected 2 - 75-90% ofheads infected 1 - >90% of heads infected most genotypes scoring 5 orbelow would typically have the majority of the spike infected

Wheat variety W030258E2 can be used as the female or the male parent inbiparental crosses in order to develop new and valuable wheat varieties.Wheat normally self-pollinates in nature. Wheat cross-pollination can beachieved by emasculating a designated female plant and pollinating thefemale plant with pollen from the designated male parent.

In order to cross pollinate one wheat plant with another to produceprogeny with a new combination of genetic traits, a method ofcross-pollination is employed. Cross-pollination is known to thoseskilled in the art. Wheat cross-pollination is achieved by emasculatingflowers of a designated female plant and pollinating the female parentwith pollen from the designated male parent. The following method wasemployed to cross-pollinate the wheat plants, but other methods can beused, or modified, as is known to those skilled in the art.

The designated female wheat plant is emasculated before its anthers shedpollen to avoid self-pollination. Emasculation is done by selecting animmature spike on the designated female parent plant that has notstarted to bloom and shed any viable pollen. Each spike consists of aseries of spikelets composed of florets which each contain one ovarywith a feathery stigma and three anthers. Typically all but the twoprimary florets are removed from each spikelet by using tweezers. Theglumes of each remaining floret can be trimmed back about 50% usingscissors to expose the immature anthers. The tweezers are used to spreadthe glumes slightly open while at the same time surrounding the anthers.The anthers can then be removed by gently grabbing and pulling them outof the flower with the tweezers in an upward motion. With skill, allthree anthers can be removed at once, but this must be confirmedvisually before moving to the next flower. Repeated attempts to removeany remaining anthers increases the risk of damage to the stigma andovary, which will greatly reduce the frequency of cross-pollination.After all the florets are emasculated on a spike, it is covered with acellophane bag to prevent pollination with stray pollen from surroundingplants. One to three days after the female spike is emasculated a maturespike that is shedding pollen is selected from the designated male plantfor cross-pollination using the approach method. The stem of the malespike is cut off at least one foot below the spike and typically theglumes of all the spikelets are trimmed back with scissors to encourageanther extrusion during pollination. The stem of the male spike isplaced in a test tube full of water, which is attached to a stickimplanted beside the emasculated female spike. The male spike is placedabove the emasculated female spike(s) in the same cellophane bag and itis permitted to shed pollen naturally over the next several days. Bywaiting a few days after emasculation, one can ensure that no anthers orviable pollen has remained in the female spike and the stigmas becomemore receptive to cross-pollination. Emasculated female spikes that areeffectively cross-pollinated by the designated male parent willtypically set 10-30 seeds per spike. Depending on the breedingobjectives, one to five spikes are typically cross-pollinated for eachcross. Spikes from the cross are hand harvested and the F1 seed from thespikes are advanced to the F1 generation. The F1 plants can be used forused for subsequent cross-pollination or they can be advanced to the F2generation for selection and further advancement. For the F2 grow out,2500 to 3500 seeds are typically planted.

DEPOSIT

Applicant has made a deposit of at least 2500 seeds of Wheat VarietyW030258E2 with the American Type Culture Collection (ATCC), 10801University Boulevard, Manassas, Va. 20110 USA, ATCC Deposit No.PTA-122841. The seeds deposited with the ATCC on Feb. 12, 2016 weretaken from the seed stock maintained by Pioneer Hi-Bred International,Inc., 7250 NW 62^(nd) Avenue, Johnston, Iowa, 50131 since prior to thefiling date of this application. Access to this seed will be availableduring the pendency of the application to the Commissioner of Patentsand Trademarks and persons determined by the Commissioner to be entitledthereto upon request. Upon allowance of any claims in the application,the Applicant will make the deposit available to the public pursuant to37 C.F.R. §1.808. This deposit of the Wheat Variety W030258E2 will bemaintained in the ATCC depository, which is a public depository, for aperiod of 30 years, or 5 years after the most recent request, or for theenforceable life of the patent, whichever is longer, and will bereplaced if it becomes nonviable during that period. Additionally,Applicant has or will satisfy all of the requirements of 37 C.F.R.§§1.801-1.809, including providing an indication of the viability of thesample upon deposit. Applicant has no authority to waive anyrestrictions imposed by law on the transfer of biological material orits transportation in commerce. Applicant does not waive anyinfringement of rights granted under this patent or under the PlantVariety Protection Act (7 USC 2321 et seq.). Unauthorized seedmultiplication is prohibited.

The foregoing invention has been described in detail by way ofillustration and example for purposes of clarity and understanding.However, it will be obvious that certain changes and modifications suchas single locus modifications and mutations, somaclonal variants,variant individuals selected from large populations of the plants of theinstant variety and the like may be practiced.

The scope of the claims should not be limited by the preferredembodiments set forth in the examples, but should be given the broadestinterpretation consistent with the description as a whole.

All publications, patents and patent applications mentioned in thespecification are indicative of the level of those skilled in the art towhich this invention pertains.

What is claimed is:
 1. A plant, plant part, seed, or plant cell of wheatvariety W030258E2, representative seed of said variety having beendeposited under ATCC accession number PTA-122841.
 2. A wheat seedproduced by crossing the plant or plant part of claim 1 with a differentwheat plant or plant part.
 3. A wheat plant produced by growing thewheat seed of claim
 2. 4. A method for producing a second wheat plantcomprising applying plant breeding techniques to the wheat plant ofclaim 3, wherein application of said techniques results in theproduction of a second wheat plant.
 5. A method for producing a progenyseed comprising crossing the wheat plant of claim 3, to a plant of wheatvariety W030258E2, representative seed of said variety having beendeposited under ATCC accession number PTA-122841 and producing a progenyseed.
 6. The method of claim 5 further comprising crossing a plant grownfrom the progeny seed of claim 5 to a plant of wheat variety W030258E2and producing a backcrossed seed.
 7. The backcrossed seed produced byclaim
 6. 8. A method for producing a double haploid wheat plant or plantpart comprising a) crossing the wheat plant of claim 3, to another plantto form haploid cells; b) doubling the chromosomes of said haploid cellsto form double haploid cells; and c) growing said double haploid cellsinto a double haploid wheat plant or plant part.
 9. A method comprisingcleaning the seed of claim
 1. 10. A method comprising conditioning theseed of claim
 1. 11. A method comprising applying a seed treatment tothe seed of claim
 1. 12. Flour produced by milling the seed of claim 1.13. A tissue culture of cells produced from the plant, plant part, seed,or plant cell of claim
 1. 14. A wheat plant regenerated from the tissueculture of claim
 13. 15. A wheat plant comprising a transgene whereinsaid wheat plant was produced by transforming the plant, plant part,seed, or cell of claim
 1. 16. A plant, plant part, seed, or plant cellof wheat variety W030258E2, representative seed of said variety havingbeen deposited under ATCC accession number PTA-122841, furthercomprising a locus conversion.
 17. The plant, plant part, seed, or plantcell of claim 16, wherein the locus conversion confers a trait selectedfrom the group consisting of male sterility, abiotic stress tolerance,altered phosphorus, altered antioxidants, altered fatty acids, alteredessential amino acids, altered carbohydrates, herbicide resistance,insect resistance and disease resistance.
 18. A wheat seed produced bycrossing the plant of claim 16 with a different wheat plant.
 19. A wheatplant produced by growing the wheat seed of claim
 18. 20. A method forproducing a second wheat plant comprising applying plant breedingtechniques to the wheat plant of claim 19, wherein application of saidtechniques results in the production of a second wheat plant.